Histological techniques for the study of granular cells during wound healing.

We have used GMA-embedded tissues sectioned 2-3 mu for the identification of granulated cells in wounded and normal macaque tissues. Mast cell granules stain brilliantly with Giemsa or with PAS counterstained with alcian blue, neutrophils stand out clearly when treated with the PAS method and macrophage granules stain with PAS as well as with basic dyes. These techniques are helpful for the identification of these cells during wound healing and during inflammatory processes.