BACKGROUND: The mobile intercellular fluid flowing to and in the lymphatics contains filtered plasma products and substances synthesized and excreted by tissue cells. Among them are signaling proteins such as cytokines, chemokines, enzymes, and growth factors. They act locally in autocrine and paracrine systems regulating cell metabolism, proliferation, and formation of the ground matrix. They play an immunoregulatory role in infections, wound healing, and tumor cell growth. METHODS AND RESULTS: In this study we measured the concentration of selected cytokines, chemokines, tissue enzymes, and growth factors in tissue fluid/lymph drained from normal human leg soft tissues. Legs exposed to infections and trauma often result in development of lymphedema. Lymph was drained from superficial calf lymphatics using microsurgical techniques. Our studies showed generally higher concentrations of cytokines, chemokines, enzymes, and growth factors in lymph than in serum. The total protein L/S ratio was 0.22, whereas that of various lymph signaling proteins ranged between 1 and 10. CONCLUSIONS: This indicates that in addition to proteins filtered from blood, local cells contribute to lymph concentration by own production, depending on the actual cell requirement. Moreover, there were major individual differences of lymph levels with simultaneous stable serum levels. This suggests existence of a local autonomous regulatory humoral mechanism in tissues, not reflected in serum.
BACKGROUND: The mobile intercellular fluid flowing to and in the lymphatics contains filtered plasma products and substances synthesized and excreted by tissue cells. Among them are signaling proteins such as cytokines, chemokines, enzymes, and growth factors. They act locally in autocrine and paracrine systems regulating cell metabolism, proliferation, and formation of the ground matrix. They play an immunoregulatory role in infections, wound healing, and tumor cell growth. METHODS AND RESULTS: In this study we measured the concentration of selected cytokines, chemokines, tissue enzymes, and growth factors in tissue fluid/lymph drained from normal human leg soft tissues. Legs exposed to infections and trauma often result in development of lymphedema. Lymph was drained from superficial calf lymphatics using microsurgical techniques. Our studies showed generally higher concentrations of cytokines, chemokines, enzymes, and growth factors in lymph than in serum. The total protein L/S ratio was 0.22, whereas that of various lymph signaling proteins ranged between 1 and 10. CONCLUSIONS: This indicates that in addition to proteins filtered from blood, local cells contribute to lymph concentration by own production, depending on the actual cell requirement. Moreover, there were major individual differences of lymph levels with simultaneous stable serum levels. This suggests existence of a local autonomous regulatory humoral mechanism in tissues, not reflected in serum.
Authors: Sidney Pestka; Christopher D Krause; Devanand Sarkar; Mark R Walter; Yufang Shi; Paul B Fisher Journal: Annu Rev Immunol Date: 2004 Impact factor: 28.527
Authors: Daniel A Cuzzone; Evan S Weitman; Nicholas J Albano; Swapna Ghanta; Ira L Savetsky; Jason C Gardenier; Walter J Joseph; Jeremy S Torrisi; Jacqueline F Bromberg; Waldemar L Olszewski; Stanley G Rockson; Babak J Mehrara Journal: Am J Physiol Heart Circ Physiol Date: 2014-03-14 Impact factor: 4.733
Authors: Talar Tokatlian; Daniel W Kulp; Andrew A Mutafyan; Christopher A Jones; Sergey Menis; Erik Georgeson; Mike Kubitz; Michael H Zhang; Mariane B Melo; Murillo Silva; Dong Soo Yun; William R Schief; Darrell J Irvine Journal: Sci Rep Date: 2018-11-08 Impact factor: 4.379