The ant genus Carebara Westwood in the Arabian Peninsula (Hymenoptera, Formicidae).

The ant genus Carebara of the Arabian Peninsula is revised. Carebara abuhurayri Sharaf & Aldawood, 2011 is synonymized under Carebara arabica Collingwood & van Harten, 2001. Carebara arabica is redescribed and a Neotype is fixed based on a specimen collected from southwestern Kingdom of Saudi Arabia. A new species, C. fayrouzae sp. n. is described from Saudi Arabia based on queens, major and minor workers. Keys to major and minor workers of the two Arabian Carebara species are given.

Introduction

The ant genus Westwood, 1840, sensu Fernández (2004), contains more than 180 described species (Bolton et al. 2006) and is distributed worldwide in the subtropics and tropics with regional taxonomic treatments available for the Palearctic (Ettershank 1966, Weber 1950, Xu 1999, Guénard and Dunn 2012), Afrotropical including Madagascar (Weber 1950, Brown 2000, Taylor 2010, Frank Azorsa, unpublished data), Neotropics (Brown 2000), Indo-Malayan (Bingham 1903), Oriental (Terayama 1996, Terayama 2009) and Australian (Taylor and Brown 1985, Shattuck 1999). Hitherto, Fernández (2004) remains the most important and comprehensive treatment of the genus for the Western Hemisphere. Five genera, Mayr,1867, Forel, 1911, Belshaw & Bolton, 1994, , Eguchi & Bui, 2007 and Sheela & Narendran, 1997 have been synonymized under (Fernández 2004).

However, very little taxonomic or biological information is available on the genus throughout its range (Bharti and Kumar 2013), especially in the Arabian Peninsula (Aldawood et al. 2011). The scarcity of information may be due to the cryptic nature of species, tiny body size, and the difficulty in collecting these ants requiring leaf litter sifting and the use of Berlese funnels for extraction. Members of the genus are subterranean and often associated with decaying wood and leaf litter (Bolton 1973, Longino 2004, Aldawood et al. 2011, Bharti and Kumar 2013).

was originally recorded from the Arabian Peninsula by Collingwood and van Harten (2001) with their description of based on minor and major workers collected from Al Kawd, near Abyan, Republic of Yemen. Ten years later, we described a new species of , Sharaf & Aldawood based on minor workers from the southwestern mountains of Kingdom of Saudi Arabia (KSA) (Aldawood et al. 2011).

Several nest series of a species very similar to were collected from four different localities in the southwestern region of KSA. Minor and major workers matched the brief original description of . In addition, two major workers of were collected from its type locality and are very similar to the major workers of . Further comparisons of this newly collected material indicated that is a synonym of .

Minor workers of another species that appeared to be undescribed were collected from Riyadh, KSA. Repeated efforts to find nests of this species that contained all castes were unsuccessful; however, a colony that contained minor and major workers and several alate queens (males unknown) was collected in eastern KSA, confirming the novelty of this taxon.

In the present work, a new species, sp. n., is described based on queens, major, and minor workers. is redescribed anddetailed new measurements are given. A Neotype of from a locality in KSA Arabia near the Republic of Yemen is designated. is synonymized with . Keys to major and minor workers of the two known Arabian Peninsula species are given.

Material and methods

Measurements and indices

TL

Total Length (HL+ Mandible length+ ML + Petiole Length + Postpetiole length + Gaster length)

HW

Head Width; maximum width of head behind eyes in full face view

HL

Head Length; maximum length of head, excluding mandibles

SL

Scape Length; excluding basal neck

EL

Eye Length; maximum diameter of eye

ML

Mesosoma Length; length of mesosoma in lateral view, from the point at which pronotum meets cervical shield to posterior base of propodeal lobes or teeth

PRW

Pronotal width, maximum width in dorsal view

PL

Petiole Length; maximum length measured in dorsal view, from anterior margin to posterior margin

PW

Petiole Width; maximum width measured in dorsal view

PPL

Postpetiole Length; maximum length measured in dorsal view

PPW

Postpetiole Width; maximum width measured in dorsal view

Indices:

SI

Scape Index (SL × 100/HW)

CI

Cephalic Index (HW × 100/HL)

All measurements are in millimeters and follow the standard measurements of Fernández (2004).

Acronyms of museums:

BMNH

Natural History Museum, London, United Kingdom

CASC

California Academy of Science Collection, San Francisco, California, USA

KSMA

King Saud University Museum of Arthropods, King Saud University, Riyadh, KSA

MCZC

Museum of Comparative Zoology, Harvard University, Cambridge, MA, USA

MHNG

Muséum ďHistoire Naturelle, Geneva, Switzerland

NHMB

Naturhistorisches Museum, Basel, Switzerland

SEMC

Division of Entomology (Snow Entomological Collections), University of Kansas Natural History Museum, Lawrence, Kansas, USA

WMLC

World Museum Liverpool, Liverpool, United Kingdom

Results

(Collingwood & van Harten, 2001)

http://species-id.net/wiki/Carebara_arabica

Figs 1 –6

Figures 1–3.

, major worker. 1 body in profile 2 body in dorsal view 3 head in full-face view (antweb.org, CASENT0906367).

Figures 4–6.

, minor worker. 4 body in profile 5 body in dorsal view 6 head in full-face view, (antweb.org, CASENT0906368).

Oligomyrmex arabica Collingwood & van Harten, 2001: 564, figs 2–4 (s. w.).

Carebara abuhurayri Sharaf & Aldawood, in

Additional material.

(3 major workers, 5 minor workers (CASENT0906368)) same data as the neotype; 6 major workers, 6 minor workers, SAUDI ARABIA, Wadi Bagara, 10.xi.2012, 18.79287°N, 42.01857°E, 436m, by leaf litter sifting (M. R. Sharaf leg.); 1 major worker, 9 minor workers, SAUDI ARABIA, Wadi Aljora, near Abadan, 12.xi.2012, 17.29263°N, 43.07010°E, 465 m, by leaf litter sifting (M. R. Sharaf leg.); 12 minor workers, SAUDI ARABIA, Fayfa, Agriculture Research Station, 6.iv.2013, 17.28671°N, 43.14390°E, 879m, (M. R. Sharaf leg.); 5 minor workers,SAUDI ARABIA, Fayfa, Agriculture Research Station, 5.iv.2013, 17.28671°N, 43.14390°E, 879m, (M. R. Sharaf leg.) [KSMA]; 1 major worker, SAUDI ARABIA, Al Bahah, Al Mukhwah, Zei Ein Archaeological Village, 19.9294°N, 41.4417°E, 741 m., 15.v.2011, (M. R. Sharaf Leg.); 1 major worker, SAUDI ARABIA, Al Bahah, Al Mukhwah, Dhi Ain Archaeological Village, 19.928°N, 41.4419°E ±50 m, 735 m., (B. L. Fisher Leg.), 23.ix.2011, Coll. Code BLF27577 [CASC].

Description.

Neotype major worker. TL 2.45, HL 0.71, HW 0.52, SL 0.26, ML 0.59, PRW 0.35, PL 0.15, PW 0.17, PPL 0.12, PPW 0.21, SI 50, CI 73.

Major workers. TL 1.77-2.76, HL 0.56-0.72, HW 0.44-0.52, SL 0.22-0.28, ML 0.49-0.63, PRW 0.29-0.35, PL 0.12-0.19, PW 0.12-0.17, PPL 0.11-0.18, PPW 0.14-0.25, SI 48-64, CI 69-80 (N=10).

Holotype major worker: TL 2.53, HL 0.75, HW 0.36, SL 0.63 (Collingwood and van Harten 2001) [Presumably lost]. (In the original description, the HW and SL for major are given wrongly as 0.36 and 0.63 respectively, from the illustration they would be ca. HW 0.55 and SL 0.30).

Major worker. (Figs 1–3) Headrectangular (HL ~ 1.38 × HW) with strongly concave posterior margin and straight parallel sides; mandibles smooth and shining; masticatory margin armed with five teeth; eyes with a single oval ommatidium; anterior clypeal margin shallowly concave; antennae ten segmented with a two segmented club; scapes very short (mean SI = 54); posterior margin of head transversally carinate and posterior corners with a pair of outgrowths, appearing as blunt teeth in lateral view. Promesonotum strongly convex; metanotal area with apparent vestigial wing bases; metanotal groove deep; propodeal spines blunt, short and broadly based; petiole distinctly broader than long in dorsal view. Postpetiole clearly broader than long and broader than petiole in dorsal view. Gaster smooth and shining. Sculpture: cephalic dorsum and area in front of eyes finely densely regularly longitudinally rugulose; the ground-sculpture a fine, dense, conspicuous granulation; lateral cephalic dorsum from the posterior margin of eyes to posterior margin of head faintly and densely granulate; promesonotum smooth and shining; anepisternum smooth and shining; katepisternum and propodeum densely, transversely and conspicuously reticulate-punctate; petiole densely irregularly reticulate; postpetiole dorsum smooth and shining. Pilosity: head hairs long and sparse; petiole with two pairs of long backward directed hairs; PageBreakPageBreakpostpetiole with three pairs of long hairs; gaster with few scattered long suberect hairs and abundant subdecumbent short hairs. Colour:concolorous brownish.

Minor workers. TL 0.99–1.13, HL 0.35–0.41, HW 0.29–0.32, SL 0.21–0.28, ML 0.31–0.34, PRW 0.17–0.19, PL 0.08–0.12, PW 0.07–0.08, PPL 0.05–0.07, PPW 0.08–09, SI 69–88, CI 74–89 (N=7).

Minor worker. (Figs 4–6) Head distinctly longer than broad (CI 74-89), with clearly convex sides and straight posterior margin; mandibles smooth and shining with relatively long yellow hairs and armed with four teeth; median portion of clypeus flat; in anterolateral view, clypeal lateral carinae strongly narrowed posteriorly between frontal lobes, then continued as a frontal triangle; eyes minute, with a single ommatidium; antennae ten segmented with a two segmented club; scapes broaden evenly from about mid-length and fail to reach head posterior margin by about one-third of the head length. Mesosoma in lateral view feebly convex; metanotal groove shallow but distinct, dorsally and laterally; propodeum obliquely angled; propodeal spiracle relatively large, circular, high and close to propodeal declivity; metapleural gland orifice prominent. Petiole longer than broad in dorsal view with short peduncle. Node of postpetiolelower than petiole and dorsally clearly convex and nearly as long as broad. Sculpture: Anterolateral sides of head very finely longitudinally striated; lower half of mesopleura, metapleura, petiole and postpetiole with areolate-rugose sculpture. Pilosity: appressed, cephalic dorsum with abundant scattered hair pits, few and short on mesosoma, petiole, postpetiole, and rare on first gastral tergite, underside of head with few short straight hairs. Clypeus with two pairs of standing hairs, central pair long and lateral pair shorter. Colour: Overall unicolorous yellow, smooth and shining.

Remarks.

A recent search conducted by the senior author and Tony Hunter (Curator of Entomology, WMLC) failed to locate any original type material of at the cited depository (Collingwood and van Harten 2001). Identification of this species has been difficult for non-specialists due to the brief original description and illustrations not indicating important diagnostic characters. Due to the apparent loss of all type material and the brief description, a Neotype from southwestern KSA is designated above for .

Following the definition of Fernández (2004), belongs to the species complex that can be recognized by the following combination of characters: the minor workers are very small; the majors with massive heads; antennae nine to eleven-segmented, with a two segmented club; mandibles armed with four or five teeth; eyes present but reduced in both minor and major workers; metanotal groove distinct; propodeum armed with triangular teeth or denticles.

Biology.

was found in Fayfa, KSA nesting in leaf litter among A. Juss. (Meliaceae), Lam (Moraceae), and Mill. (Rosaceae) trees and coexisting with an unidentified termite species. The other nest series from Wadi Bagara was found nesting in loose soil under roots of a Poaceae and near and Giant Milkweed, (Aiton) (Asclepiadaceae). Other ants’ associates included (Mayr, 1904); F., 1793, and sp. An interesting observation concerning a nest series from Zei Ein Archaeological Village included major workers, an uncommon phenomenon as compared to other nests found in Wadi Khat, Wadi Bagara, Wadi Aljora, and Fayfa. Two major workers were collected by digging in soil. Numerous minor workers were observed foraging above ground and exiting and entering tiny nest entrances in compacted humid clay soil. No major workers were observed foraging above ground.

Sharaf sp. n.

http://zoobank.org/4E99379F-D985-4582-872A-A2B98D01F547

http://species-id.net/wiki/Carebara_fayrouzae

Figs 7 –15

Figures 7–9.

sp. n., major worker 7 body in profile 8 body in dorsal view 9 head in full-face view (antweb.org, CASENT0280975).

Figures 13–15.

sp. n., Queen 13 body in profile 14 body in dorsal view 15 head in full-face view, (antweb.org, CASENT0906362).

Type material.

Holotype major worker. SAUDI ARABIA, Al Qatif, El Naft, Eastern Province, 26.51028°N, 49.96889°E, 30 m. 23.iii.2012 (M. R. Sharaf Leg.) (MRS0066); King Saud University Museum of Arthropods (KSMA), College of Food and Agriculture Sciences, King Saud University, Riyadh, KSA.

Paratypes. 28 minor workers (CASENT0280994), 16 major workers (CASENT0280975), 4 queens (CASENT0906362) with same locality and data as the holotype (KSMA); a single paratype specimen of minor and major workers, and queen are deposited in MHNG; NHMB; CASC; MCZC; SEMC; WMLC; BMNH; 2 minor workers, SAUDI ARABIA, Riyadh, Alhaeir, 24.59214°N, 46.74522°E, 24.iii.2009, lemon soil, Berlese funnel (Acarology lab team at Department of Plant Protection, KSU Leg.); 4 minor workers, SAUDI ARABIA, Riyadh, iii.1989, Soil fauna (no collector data); 2 minor workers, SAUDI ARABIA, N. Riyadh, Ammaryia, 5.iii.2010, 24.806402°N, 46.428845°E, 681m, Lettuce soil fauna, Berlese funnel (Acarology lab team at Department of Plant Protection, KSU Leg.) These paratypes are in KSMA; 1 minor worker, SAUDI ARABIA, Riyadh, Mezahmia, Rawdat Kharara, 24.38931°N, 46.24211°E, 712 m. 30.i.2011, (M. R. Sharaf Leg.) (CASC); 1 minor worker, SAUDI ARABIA, Riyadh, Al Rowayda, 25.88016°N, 45.11563°E, 22.ii.2009, (M. R. Sharaf Leg.) (CASC) (All are paratypes).

Holotype major worker. TL 3.62, HL 1.10, HW 0.72, SL 0.37, EL 0.05, ML 0.87, PRW 0.50, PL0.20, PW 0.25, PPL 0.20, PPW 0.27, SI 51, CI 65.

Paratype major workers. TL 3.27–5.00, HL 1.00–1.20, HW 0.72–0.87, SL 0.32–0.42, EL 0.05, ML0.85–1.12, PRW 0.50–0.60, PL 0.20–0.27, PW 0.22–0.30, PPL 0.20–0.27, PPW 0.27–0.40, SI 38–53, CI 65–78 (N=15).

Major worker. (Figs 7–9) Head longer than broad (HL = 1.5 × HW), with feebly concave posterior margin, rounded posterior corners and parallel sides; masticatory margin of mandibles armed with four teeth; antennae nine-segmented; antennal scapes very short; clypeus narrow and with weakly concave anterior margin; eyes very tiny (in some individuals eyes absent); ocelli absent. Mesosoma in profile with distinct promesonotal suture; promesonotum feebly concave; metanotum small and narrow; dorsal face of propodeum continuously sloping and curving into declivity without spine or PageBreakPageBreakangle. Petiole in dorsal view little broader than long; petiolar peduncle short; petiolar ventral process distinct. Postpetiole in dorsal view nearly twice as broad as long, with acute lateral angles. Postpetiole in profile with distinct ventral process. Sculpture: Body smooth and glossy, anterior part of head finely, longitudinally rugulose, area between meso- and metapleura finely cross-ribbed. Pilosity: Head dorsum with scattered short hairs, rest of body with longer dense, yellow hairs. Colour:Bicolored species, head and mesosoma brownish, petiole and postpetiole brownish yellow, antennae, legs and gaster clear yellow.

Paratype minor workers. TL 1.66–1.94, HL 0.44–0.51, HW 0.29–0.44, SL 0.25–0.31, EL 0.007, ML0.49–0.53, PRW 0.22–0.31, PL 0.09–0.17, PW 0.09–0.14, PPL 0.07–0.09, PPW 0.09–0.12, SI 59–79, CI 78–100 (N=15).

Minor worker. (Figs 10–12) Head distinctly longer than broad, with straight posterior margin and parallel sides; masticatory margin of mandibles armed with four teeth; antennae nine-segmented; scapes when laid back from their insertions fail to reach posterior margin of head by about one third of head width; anterior clypeal margin nearly straight; eyes with only one ommatidium (absent in some individuals). Mesosoma nearly flat in profile; promesonotal suture indistinct; metanotal groove distinct; propodeal dorsum meeting declivity in a continuous curve; propodeal spiracle in profile high and located above midline of propodeum. Petiole in profile shortly pedunculate, with blunt ventral process and in dorsal view slightly longer than broad. Node of postpetiole in dorsal view clearly broader than long and in profile distinctly lower than petiolar node. Colour:Unicolorous clear yellow, hairy, smooth and moderately shining.

Figures 10–12.

sp. n., minor worker 10 body in profile 11 body in dorsal view 12 head in full-face view (antweb.org, CASENT0280994).

Paratype queens. TL 9.75–10.75, HL 1.10–1.35, HW 1.45–1.50, SL 0.60–0.90, EL 0.35-0.45, ML3.25–3.50, PL 0.50–0.75, PW 0.65–0.70, PPL 0.50–0.60, PPW 0.85–1.00, SI 40–62, CI 107–136 (N=3).

Queen. (Figs 13–15) Body enormous, notably larger than minor and major workers. Head triangular, broader than long (HW = 1.2 × HL), with straight posterior margin and strongly convex lateral margins; masticatory margin of mandibles armed with four teeth; antennae nine-segmented; antennal scapes when laid back from their insertions reach level of posterior margin of eyes; anterior clypeal margin convex, eyes large and multifaceted (about 0.27 × HW); ocelli present. Mesosoma robust, pronotum not exposed above, lying entirely beneath the mesonotum; propodeum unarmed; remaining characters modified as in myrmicine queens. Petiole in dorsal view longer than broad. Postpetiole very broadly attached to gaster and node in dorsal view distinctly broader than long. Sculpture: Anterior half of cephalic dorsum with fine longitudinal striations extend to before posterior level of eyes; median portion of clypeus, posterior half of head, mesosoma and gaster smooth and shining, petiole and postpetiole superficially and finely shagreenate. Pilosity: Whole body covered with abundant, long, yellow hairs. Colour: Uniformly black, funiculi, tibiae and tarsi blackish brown.

Male. Not known.

Comparative notes.

This new species is the second member of the genus recorded from the Arabian Peninsula. According to Fernández (2004), PageBreakPageBreakPageBreak belongs to species complex with nine antennal segments and unarmed propodeum. It does not resemble any of the American species (Fernández, pers. comm.), or Madagascar species (Azorsa, pers. comm.) and majors are easily distinguished from of the group, by the nine segmented antennae, the absence of cephalic posterolateral teeth and the smooth and shining cephalic dorsum. Superficially, resembles (Pisarski, 1970) from Afghanistan in regard to the smooth and shining habitus and the absence of cephalic posterolateral teeth. can be easily distinguished by the bicoloured major workers, the nine-segmented antennae and the node of postpetiole which is broader than petiolar node in dorsal view. In , major workers are concolorous yellow, antennae ten-segmented and node of postpetiole as broad as petiolar node in dorsal view.

This new species was found nesting in leaf litter under a large almond tree, Batsch (Rosaceae) in a fenced area of a farm. The soil was composed of two layers, a thin upper clay layer organically enriched where most specimens were found foraging, and a lower thicker layer of loose sand where few specimens were found. Two beetle species were found coexisting with the ants, (Silvanidae) and (Cryptophagidae).

Etymology.

This new species is dedicated to Fayrouz Sharaf (the daughter of the senior author).

Discussion

The Arabian Peninsula is located on the line of contact between three major zoogeographical regions, the Palearctic, the Afrotropical and the Oriental regions; therefore, it is reasonable that it shares some faunal affinities with the mentioned regions. The central and eastern areas of the Arabian Peninsula belong to the Palearctic region with Eremic influence (Uvarov 1938, Büttiker and Wittmer 1979, Greathead 1980 and Larsen 1984, Sharaf et al. 2013); the southwestern region (Mountains of Al Sarawat and Asir to Yemen) belong to the Afrotropical region (Bodenheimer 1937, Nayman 1972, Zohary 1973, Sharaf et al. 2012 and Elhawagryi et al. 2013); whereas the north east, particularly near Iraqi and Kuwaiti borders and along mountains of eastern Oman belong to the Oriental region (Büttiker and Wittmer 1979).

In spite of the mentioned faunal affinities, the probability of to be an introduced or invasive species, is unlikely for two reasons, first, the new species is well represented very much inland in central Arabian deserts (Riyadh and adjacent areas), second, it does not resemble any of the Asian species, (e.g. the Indian species) (Bharti and Kumar 2013). The poverty of knowledge of the genus for adjacent countries east of the Arabian Gulf (e.g. Iran, Pakistan, etc.) makes the hypothesis difficult to test completely.

At present, two species of PageBreak are now known from the Arabian Peninsula, of the concinna species complex from the Republic of Yemen and KSA and sp. n. of the lignata species complex from KSA. The subterranean nature and nesting habit in decaying wood or leaf litter of this ant group (Bolton 1973, Hölldobler and Wilson 1990, Bharti and Kumar 2013) no doubt has resulted in a paucity of information available about its ecology and biology (Aldawood et al. 2011, Bharti and Kumar 2013). It seems likely that is a species of mountainous ecosystems of southwestern KSA and Republic of Yemen. In contrast, was found in desert ecosystems of the central and eastern regions of KSA.

1	Smallerspecies (TL 1.77–2.76); antennae 10-segmented; concolorous brownish, antennae and legs yellowish; posterior margin of head strongly concave and posterior corners with a pair of teeth or horns, appearing blunt in profile; cephalic dorsum dull, with fine, dense, regular and longitudinal rugulae; lateral margins of postpetiole in dorsal view rounded	Carebara arabica
–	Largerspecies (TL 3.27–5.00); antennae 9-segmented; bicolored, head and mesosoma brownish, petiole and postpetiole brownish yellow, antennae, legs and gaster clear yellowish; posterior margin of head feebly concave and posterior corners rounded, without teeth or horns; cephalic dorsum smooth and shining except anterior part of head finely, longitudinally rugulose; lateral margins of postpetiole distinctly angular in dorsal view	Carebara fayrouzae sp. n.

1	Antennae 10-segmented; eyes minute, with a single ommatidium (present in all individuals); body pilosity subdecumbent or appressed and much scarce; anterolateral sides of head very finely longitudinally striated; lower halves of mesopleuron, metapleuron, petiole and postpetiole areolate-rugose; propodeal dorsum nearly half as long as propodeal declivity in profile	Carebara arabica
–	Antennae 9-segmented; eyes as rudimentary ommatidium (absent in some individuals); body pilosity erect to suberect and dense; entire body smooth without any type of surface sculpture; propodeal dorsum as long as declivity, appearing as a continuous curve in profile	Carebara fayrouzae sp. n.