Jan Bures1, Jaroslav Kvetina2, Michal Pavlik3, Martin Kunes4, Marcela Kopacova2, Stanislav Rejchrt2, Daniel Jun5, Martina Hrabinova5, Kamil Kuca4, Ilja Tachecí2. 1. 2nd Department of Internal Medicine - Gastroenterology, Charles University Faculty of Medicine and University Hospital, Hradec Králové, Czech Republic. bures@lfhk.cuni.cz. 2. 2nd Department of Internal Medicine - Gastroenterology, Charles University Faculty of Medicine and University Hospital, Hradec Králové, Czech Republic. 3. Department of Teaching Support, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic. 4. Biomedical Research Center, University Hospital, Hradec Králové, Czech Republic. 5. Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
Abstract
OBJECTIVES: Organophosphorus compounds represent nerve agents, pesticides and several industrial compounds. Treatment after exposure to organophosphates involves the use of parasympatolytics, acetylcholinesterase (AChE) reactivators/modulators and anticonvulsive drugs. Wider clinical use of several AChE reactivators/modulators might be limited because of possible side effects, including gastrointestinal toxicity. In this study we evaluated the effect of paraoxon and an AChE reactivator (HI-6) on the gastric myoelectric activity in experimental pigs. METHODS: Six female experimental pigs (mean weight 33 kg) entered the study. Intramuscular paraoxon (1.5 g) was administrated after the baseline gastric electrogastrography (EGG) recording, followed by HI-6 dimethansulphonate (1.5 g i.m.) 10 min. later. A further ten 15-minute-interval EGG recordings were performed. Running spectral analysis was used for the elemental evaluation of the EGG. The results were expressed as dominant frequency of slow waves at all intervals of EGG recordings. EGG power analysis was performed in all animals. RESULTS: Paraoxon induced a non-significant decrease of dominant frequency (2.8±0.6 vs. 2.6±0.5 cycles per min.; p=0.092). Subsequent administration of HI-6 normalised dominant frequency to basal values and increased it significantly within the subsequent 30 minutes (3.0±0.4; p<0.001). Paraoxon administration did not influence the power (within a 10-minute exposure). However, the amplitudes increased significantly 90 minutes after administration of HI-6 (819±109 vs. 5054±732 μV2; p<0.001). CONCLUSIONS: AChE reactivator HI-6 blocked the gastric effect of paraoxon significantly. Subsequent myoelectric changes in the dominant frequency and power were executed by HI-6. The effect of paraoxon was non-significant.
OBJECTIVES:Organophosphorus compounds represent nerve agents, pesticides and several industrial compounds. Treatment after exposure to organophosphates involves the use of parasympatolytics, acetylcholinesterase (AChE) reactivators/modulators and anticonvulsive drugs. Wider clinical use of several AChE reactivators/modulators might be limited because of possible side effects, including gastrointestinal toxicity. In this study we evaluated the effect of paraoxon and an AChE reactivator (HI-6) on the gastric myoelectric activity in experimental pigs. METHODS: Six female experimental pigs (mean weight 33 kg) entered the study. Intramuscular paraoxon (1.5 g) was administrated after the baseline gastric electrogastrography (EGG) recording, followed by HI-6 dimethansulphonate (1.5 g i.m.) 10 min. later. A further ten 15-minute-interval EGG recordings were performed. Running spectral analysis was used for the elemental evaluation of the EGG. The results were expressed as dominant frequency of slow waves at all intervals of EGG recordings. EGG power analysis was performed in all animals. RESULTS:Paraoxon induced a non-significant decrease of dominant frequency (2.8±0.6 vs. 2.6±0.5 cycles per min.; p=0.092). Subsequent administration of HI-6 normalised dominant frequency to basal values and increased it significantly within the subsequent 30 minutes (3.0±0.4; p<0.001). Paraoxon administration did not influence the power (within a 10-minute exposure). However, the amplitudes increased significantly 90 minutes after administration of HI-6 (819±109 vs. 5054±732 μV2; p<0.001). CONCLUSIONS:AChE reactivator HI-6 blocked the gastric effect of paraoxon significantly. Subsequent myoelectric changes in the dominant frequency and power were executed by HI-6. The effect of paraoxon was non-significant.
Authors: Francine S Katz; Stevan Pecic; Timothy H Tran; Ilya Trakht; Laura Schneider; Zhengxiang Zhu; Long Ton-That; Michal Luzac; Viktor Zlatanic; Shivani Damera; Joanne Macdonald; Donald W Landry; Liang Tong; Milan N Stojanovic Journal: Chembiochem Date: 2015-09-09 Impact factor: 3.164
Authors: Jan Bures; Jaroslav Kvetina; Vera Radochova; Ilja Tacheci; Eva Peterova; David Herman; Rafael Dolezal; Marcela Kopacova; Stanislav Rejchrt; Tomas Douda; Vit Sestak; Ladislav Douda; Jana Zdarova Karasova Journal: PLoS One Date: 2020-01-24 Impact factor: 3.240
Authors: Jan Bures; Ilja Tacheci; Jaroslav Kvetina; Vera Radochova; Darina Kohoutova; Martin Valis; Stanislav Rejchrt; Veronika Knoblochova; Jana Zdarova Karasova Journal: Pharmaceuticals (Basel) Date: 2021-06-18