Virus-induced signaling influences endosome trafficking, virus entry, and replication.

Viruses are obligate intracellular pathogens that interact with host cell machinery for enabling entry, replication, and spread. This chapter describes methods for studying the interaction of viruses with host cell signaling pathways and surface receptors during cellular infection, with an emphasis on protein kinases. We also describe how use of immunofluorescence confocal microscopy for imaging virus-host interactions provides a powerful approach for obtaining structural correlations that extend results of immunological and biochemical assays.