BACKGROUND/AIMS: Bone marrow-derived cells (BMDCs) reduced mechanical and histologic changes in the lung in a murine model of silicosis, but these beneficial effects did not persist in the course of lung injury. We hypothesized that repeated administration of BMDCs may decrease lung inflammation and remodeling thus preventing disease progression. METHODS: One hundred and two C57BL/6 mice were randomly divided into SIL (silica, 20 mg intratracheally [IT]) and control (C) groups (saline, IT). C and SIL groups were further randomized to receive BMDCs (2×10(6) cells) or saline IT 15 and 30 days after the start of the protocol. RESULTS: By day 60, BMDCs had decreased the fractional area of granuloma and the number of polymorphonuclear cells, macrophages (total and M1 phenotype), apoptotic cells, the level of transforming growth factor (TGF)-β' and types I and III collagen fiber content in the granuloma. In the alveolar septa, BMDCs reduced the amount of collagen and elastic fibers, TGF-β, and the number of M1 and apoptotic cells. Furthermore, interleukin (IL)-1β, IL-1R1, caspase-3 mRNA levels decreased and the level of IL-1RN mRNA increased. Lung mechanics improved after BMDC therapy. The presence of male donor cells in lung tissue was not observed using detection of Y chromosome DNA. CONCLUSION: repeated administration of BMDCs reduced inflammation, fibrogenesis, and elastogenesis, thus improving lung mechanics through the release of paracrine factors.
BACKGROUND/AIMS: Bone marrow-derived cells (BMDCs) reduced mechanical and histologic changes in the lung in a murine model of silicosis, but these beneficial effects did not persist in the course of lung injury. We hypothesized that repeated administration of BMDCs may decrease lung inflammation and remodeling thus preventing disease progression. METHODS: One hundred and two C57BL/6 mice were randomly divided into SIL (silica, 20 mg intratracheally [IT]) and control (C) groups (saline, IT). C and SIL groups were further randomized to receive BMDCs (2×10(6) cells) or saline IT 15 and 30 days after the start of the protocol. RESULTS: By day 60, BMDCs had decreased the fractional area of granuloma and the number of polymorphonuclear cells, macrophages (total and M1 phenotype), apoptotic cells, the level of transforming growth factor (TGF)-β' and types I and III collagen fiber content in the granuloma. In the alveolar septa, BMDCs reduced the amount of collagen and elastic fibers, TGF-β, and the number of M1 and apoptotic cells. Furthermore, interleukin (IL)-1β, IL-1R1, caspase-3 mRNA levels decreased and the level of IL-1RN mRNA increased. Lung mechanics improved after BMDC therapy. The presence of male donor cells in lung tissue was not observed using detection of Y chromosome DNA. CONCLUSION: repeated administration of BMDCs reduced inflammation, fibrogenesis, and elastogenesis, thus improving lung mechanics through the release of paracrine factors.
Authors: Marcelo M Morales; Sérgio A L Souza; Luiz Paulo Loivos; Marina A Lima; Amir Szklo; Leandro Vairo; Taís H K Brunswick; Bianca Gutfilen; Miquéias Lopes-Pacheco; Alberto J Araújo; Alexandre P Cardoso; Regina C Goldenberg; Patricia R M Rocco; Lea M B Fonseca; José R Lapa e Silva Journal: BMC Pulm Med Date: 2015-06-11 Impact factor: 3.317
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Authors: Fernanda Ferreira Cruz; Lucas Felipe Bastos Horta; Lígia de Albuquerque Maia; Miquéias Lopes-Pacheco; André Benedito da Silva; Marcelo Marco Morales; Cassiano Felippe Gonçalves-de-Albuquerque; Christina Maeda Takiya; Hugo Caire de Castro-Faria-Neto; Patricia Rieken Macedo Rocco Journal: PLoS One Date: 2016-01-20 Impact factor: 3.240
Authors: Priscila J Carneiro; Amanda L Clevelario; Gisele A Padilha; Johnatas D Silva; Jamil Z Kitoko; Priscilla C Olsen; Vera L Capelozzi; Patricia R M Rocco; Fernanda F Cruz Journal: Front Physiol Date: 2017-03-15 Impact factor: 4.566