Deshraj Jain1, Prabha Shakya. 1. Department of Prosthodontics, Government College of Dentistry, Indore, Madhya Pradesh, India.
Abstract
BACKGROUND: Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this thorough study, we evaluate the effect of Delmopinol application on Candida albicans adherence on heat cured denture base acrylic resin. MATERIALS AND METHODS: A total of 40 specimens of heat-cured acrylic resin were made, 20 specimens were contaminated before Delmopinol treatment and 20 specimens were contaminated after Delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μL of each tube was then collected and inoculated into agar sabouraud plates containing 500 mMol/L of sucrose, which was incubated for 24 hours at 37°C, in order to check microbial growth. RESULTS: Mean of the turbidity Degree of contamination after Delmopinol application (1440.80, colony-forming unit [CFU] [×10 6 /ml]) was significantly higher than the mean of the turbidity degree of contamination before Delmopinol application (550.85 CFU [×10 6 /ml]). CONCLUSION: Heat-cured acrylic resin shows greater reduction in adherence of Candida albicans by contamination after Delmopinol application as compared with contamination before Delmopinol application.
BACKGROUND: Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this thorough study, we evaluate the effect of Delmopinol application on Candida albicans adherence on heat cured denture base acrylic resin. MATERIALS AND METHODS: A total of 40 specimens of heat-cured acrylic resin were made, 20 specimens were contaminated before Delmopinol treatment and 20 specimens were contaminated after Delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μL of each tube was then collected and inoculated into agar sabouraud plates containing 500 mMol/L of sucrose, which was incubated for 24 hours at 37°C, in order to check microbial growth. RESULTS: Mean of the turbidity Degree of contamination after Delmopinol application (1440.80, colony-forming unit [CFU] [×10 6 /ml]) was significantly higher than the mean of the turbidity degree of contamination before Delmopinol application (550.85 CFU [×10 6 /ml]). CONCLUSION: Heat-cured acrylic resin shows greater reduction in adherence of Candida albicans by contamination after Delmopinol application as compared with contamination before Delmopinol application.
Authors: Julyana de Araújo Oliveira; Ingrid Carla Guedes da Silva; Leonardo Antunes Trindade; Edeltrudes Oliveira Lima; Hugo Lemes Carlo; Alessandro Leite Cavalcanti; Ricardo Dias de Castro Journal: Evid Based Complement Alternat Med Date: 2014-12-09 Impact factor: 2.629