AIMS: To evaluate a novel exopolysaccharide (EPS1) from the recently described haloalkaliphilic, thermophilic Bacillus licheniformis strain T14, isolated from a shallow hydrothermal vent of Panarea Island (Italy), for its antiviral and immunomodulatory effects against herpes simplex virus type 2 (HSV-2). METHODS AND RESULTS: EPS1-T14 hindered the HSV-2 replication in human peripheral blood mononuclear cells (PBMC) but not in WISH cells, indicating that cell-mediated immunity was involved in the antiviral activity. High levels of Th1-type cytokines were detected in supernatants of EPS1-treated PBMC, while Th2-type cytokines were not induced. CONCLUSIONS: The novel EPS1-T14 is a water-soluble, noncytotoxic exopolymer able to stimulate the immune response and thus contribute to the antiviral immune defence, acting as immunomodulator. SIGNIFICANCE AND IMPACT OF THE STUDY: The exopolysaccharide produced by B. licheniformis strain T14, stimulator of Th1 cell-mediated immunity, could be used as therapy in immunocompromised host.
AIMS: To evaluate a novel exopolysaccharide (EPS1) from the recently described haloalkaliphilic, thermophilic Bacillus licheniformis strain T14, isolated from a shallow hydrothermal vent of Panarea Island (Italy), for its antiviral and immunomodulatory effects against herpes simplex virus type 2 (HSV-2). METHODS AND RESULTS:EPS1-T14 hindered the HSV-2 replication in human peripheral blood mononuclear cells (PBMC) but not in WISH cells, indicating that cell-mediated immunity was involved in the antiviral activity. High levels of Th1-type cytokines were detected in supernatants of EPS1-treated PBMC, while Th2-type cytokines were not induced. CONCLUSIONS: The novel EPS1-T14 is a water-soluble, noncytotoxic exopolymer able to stimulate the immune response and thus contribute to the antiviral immune defence, acting as immunomodulator. SIGNIFICANCE AND IMPACT OF THE STUDY: The exopolysaccharide produced by B. licheniformis strain T14, stimulator of Th1 cell-mediated immunity, could be used as therapy in immunocompromised host.
Authors: G Rodríguez-Valdez; R Romero-Geraldo; G Medina-Basulto; M Reyes-Becerril; C Angulo Journal: Indian J Microbiol Date: 2022-02-05 Impact factor: 2.461
Authors: Concetta Gugliandolo; Antonio Spanò; Teresa L Maugeri; Annarita Poli; Adriana Arena; Barbara Nicolaus Journal: Microorganisms Date: 2015-08-21