Literature DB >> 24352998

Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves.

Bobby L Boyanton1, Amro Almradi, Mark J Espy, Anne E Prada, John P Gibson, Bobbi S Pritt.   

Abstract

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

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Mesh：

Year:  2013        PMID： 24352998      PMCID： PMC3957778          DOI： 10.1128/JCM.03062-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR.

Authors:  M J Espy; J R Uhl; P S Mitchell; J N Thorvilson; K A Svien; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Failure to genotype herpes simplex virus by real-time PCR assay and melting curve analysis due to sequence variation within probe binding sites.

Authors:  Trevor P Anderson; Anja M Werno; Kirsten A Beynon; David R Murdoch
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

3.  Sequencing and resolution of amplified herpes simplex virus DNA with intermediate melting curves as genotype 1 or 2 by LightCycler PCR assay.

Authors:  Nicolas C Issa; Mark J Espy; James R Uhl; Thomas F Smith
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

4.  Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture.

Authors:  Julie Burrows; Andreas Nitsche; Belinda Bayly; Elise Walker; Geoff Higgins; TuckWeng Kok
Journal:  BMC Microbiol       Date:  2002-06-09       Impact factor: 3.605
  4 in total
  2 in total

1.  Testing for Herpes Simplex Virus in Low-Volume Cerebrospinal Fluid Samples: Comparison of Three Protocols To Optimize Detection.

Authors:  Mark J Espy; Cole L Irish; Matthew J Binnicker
Journal:  J Clin Microbiol       Date:  2015-12       Impact factor: 5.948

2.  Simultaneous detection of herpes simplex virus 1 and 2 in the cerebrospinal fluid of a patient with seizures and encephalitis.

Authors:  Neil W Anderson; William Sistrunk; Matthew J Binnicker
Journal:  J Clin Microbiol       Date:  2014-10-29       Impact factor: 5.948
  2 in total

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