AIM: To examine the performance of an injectable gel graft made of transglutaminase (Tg)-crosslinked gelatin gel with BMP-2 (BMP-2-Tg-Gel) for bone defect repair in animal models. MATERIALS & METHODS: BMP-2 mixed with gelatin gel was crosslinked using Tg. The release of tethered BMP-2 through autocrine and paracrine pathways was demonstrated by using C2C12 and NIH 3T3 cells, respectively. BMP-2-Tg-Gel was injected into the induced cranial defect site. After 14 days, the sample was removed for x-ray imaging and histological evaluation. RESULTS: Our in vivo results demonstrated that the injectable Tg-Gel with its osteoconductivity and controllable BMP-2 activity induced bone formation in our rat models when tethered with BMP-2. CONCLUSION: Tg-Gel as an injectable functional bone graft may enable the use of minimally invasive surgical procedures to treat irregular-shaped bone defects. Furthermore, this novel approach is capable of incorporating and controlling the release of therapeutic agents that may advance the science of tissue regeneration.
AIM: To examine the performance of an injectable gel graft made of transglutaminase (Tg)-crosslinked gelatin gel with BMP-2 (BMP-2-Tg-Gel) for bone defect repair in animal models. MATERIALS & METHODS: BMP-2 mixed with gelatin gel was crosslinked using Tg. The release of tethered BMP-2 through autocrine and paracrine pathways was demonstrated by using C2C12 and NIH 3T3 cells, respectively. BMP-2-Tg-Gel was injected into the induced cranial defect site. After 14 days, the sample was removed for x-ray imaging and histological evaluation. RESULTS: Our in vivo results demonstrated that the injectable Tg-Gel with its osteoconductivity and controllable BMP-2 activity induced bone formation in our rat models when tethered with BMP-2. CONCLUSION: Tg-Gel as an injectable functional bone graft may enable the use of minimally invasive surgical procedures to treat irregular-shaped bone defects. Furthermore, this novel approach is capable of incorporating and controlling the release of therapeutic agents that may advance the science of tissue regeneration.
Authors: Jerald E Dumas; Pamela B BrownBaer; Edna M Prieto; Teja Guda; Robert G Hale; Joseph C Wenke; Scott A Guelcher Journal: Biomed Mater Date: 2012-03-29 Impact factor: 3.715
Authors: L Ambrosio; V Guarino; V Sanginario; P Torricelli; M Fini; M P Ginebra; J A Planell; R Giardino Journal: Biomed Mater Date: 2012-03-29 Impact factor: 3.715
Authors: Christopher R Kinsella; Michael R Bykowski; Alexander Y Lin; James J Cray; Emily L Durham; Darren M Smith; Gary E DeCesare; Mark P Mooney; Gregory M Cooper; Joseph E Losee Journal: Plast Reconstr Surg Date: 2011-05 Impact factor: 4.730
Authors: Maxwell A Serowoky; Stephanie T Kuwahara; Shuwan Liu; Venus Vakhshori; Jay R Lieberman; Francesca V Mariani Journal: NPJ Regen Med Date: 2022-05-17