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Literature DB >> 2434932

Catalytic activity of the nucleic acid component of the 1,4-alpha-glucan branching enzyme from rabbit muscles.

T A Shvedova, G A Korneeva, V A Otroshchenko, T V Venkstern.

Abstract

2.5 S RNA, the nucleic acid component of the 1,4-alpha-D-glucan: 1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase from rabbit muscles, devoid of any protein, catalyses the branching reaction, as does the holoenzyme. The conclusion is drawn that 2.5 S RNA is a ribozyme. To get an insight into the significance of different parts of the molecule for the catalytic activity of 2.5 S RNA, a large fragment isolated from its partial RNAase A digest was investigated. This fragment which proved to be the middle part of polyribonucleotide chain containing all modified nucleotides exerts some catalytic activity, too.

Entities: Chemical Species

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Year: 1987 PMID： 2434932 PMCID： PMC340578 DOI： 10.1093/nar/15.4.1745

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

Catalytic activity of the nucleic acid component of the 1,4-alpha-glucan branching enzyme from rabbit muscles.

1. [Studies on active forms of amylose isomerase].

2. [Nucleotide composition and digestion by nucleases of RNA from muscle amylose isomerase].

3. Catalytic activity of an RNA molecule prepared by transcription in vitro.

4. First true RNA catalyst found.

5. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.

6. [Primary structure and protein-binding portion of a polyribonucleotide isolated from branching enzyme].

7. Primary structure of the nucleic acid from the 1,4-alpha-glucan branching enzyme.

8. [Study of 2.5S RNA of 1,4-alpha-glucan branching enzyme by fluorescent methods using ethidium bromide].

9. On the participation of lowpolymeric (3S) ribonucleic acid in the branching reaction of polysaccharides.

10. [Structure-function study of 1,4-alpha-glucan branching enzyme by limited trypsin treatment].

1. Metabolic complexity in the RNA world and implications for the origin of protein synthesis.

2. Origin of genetically encoded protein synthesis: a model based on selection for RNA peptidation.