Literature DB >> 24344681

A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies.

Robert N Wilkinson1, Stone Elworthy2, Philip W Ingham2, Fredericus J M van Eeden2.   

Abstract

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

Entities:  

Keywords:  PCR; embryo; fin clip; genotyping; zebrafish

Mesh：

Year:  2013        PMID： 24344681     DOI： 10.2144/000114116

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  26 in total

1.  Nfe2 is dispensable for early but required for adult thrombocyte formation and function in zebrafish.

Authors:  Megan S Rost; Ilya Shestopalov; Yang Liu; Andy H Vo; Catherine E Richter; Sylvia M Emly; Francesca G Barrett; David L Stachura; Michael Holinstat; Leonard I Zon; Jordan A Shavit
Journal:  Blood Adv       Date:  2018-12-11

2.  nox2/cybb Deficiency Affects Zebrafish Retinotectal Connectivity.

Authors:  Cory J Weaver; Aslihan Terzi; Haley Roeder; Theodore Gurol; Qing Deng; Yuk Fai Leung; Daniel M Suter
Journal:  J Neurosci       Date:  2018-05-23       Impact factor: 6.167

3.  SRP54 mutations induce congenital neutropenia via dominant-negative effects on XBP1 splicing.

Authors:  Christoph Schürch; Thorsten Schaefer; Joëlle S Müller; Pauline Hanns; Marlon Arnone; Alain Dumlin; Jonas Schärer; Irmgard Sinning; Klemens Wild; Julia Skokowa; Karl Welte; Raphael Carapito; Seiamak Bahram; Martina Konantz; Claudia Lengerke
Journal:  Blood       Date:  2021-03-11       Impact factor: 22.113

4.  "But It's Just a Fish": Understanding the Challenges of Applying the 3Rs in Laboratory Aquariums in the UK.

Authors:  Reuben Message; Beth Greenhough
Journal:  Animals (Basel)       Date:  2019-12-03       Impact factor: 2.752

5.  Analysis of factor V in zebrafish demonstrates minimal levels needed for early hemostasis.

Authors:  Angela C Weyand; Steve J Grzegorski; Megan S Rost; Kari I Lavik; Allison C Ferguson; Marzia Menegatti; Catherine E Richter; Rosanna Asselta; Stefano Duga; Flora Peyvandi; Jordan A Shavit
Journal:  Blood Adv       Date:  2019-06-11

6.  Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function.

Authors:  Yang Liu; Colin A Kretz; Morgan L Maeder; Catherine E Richter; Philip Tsao; Andy H Vo; Michael C Huarng; Thomas Rode; Zhilian Hu; Rohit Mehra; Steven T Olson; J Keith Joung; Jordan A Shavit
Journal:  Blood       Date:  2014-04-29       Impact factor: 22.113

7.  Genome editing of factor X in zebrafish reveals unexpected tolerance of severe defects in the common pathway.

Authors:  Zhilian Hu; Yang Liu; Michael C Huarng; Marzia Menegatti; Deepak Reyon; Megan S Rost; Zachary G Norris; Catherine E Richter; Alexandra N Stapleton; Neil C Chi; Flora Peyvandi; J Keith Joung; Jordan A Shavit
Journal:  Blood       Date:  2017-06-02       Impact factor: 22.113

8.  High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping.

Authors:  Ceres Kosuta; Kate Daniel; Devon L Johnstone; Kevin Mongeon; Kevin Ban; Sophie LeBlanc; Stuart MacLeod; Karim Et-Tahiry; Marc Ekker; Alex MacKenzie; Izabella Pena
Journal:  J Vis Exp       Date:  2018-06-29       Impact factor: 1.355

9.  Loss of fibrinogen in zebrafish results in an asymptomatic embryonic hemostatic defect and synthetic lethality with thrombocytopenia.

Authors:  Zhilian Hu; Kari I Lavik; Yang Liu; Andy H Vo; Catherine E Richter; Jorge Di Paola; Jordan A Shavit
Journal:  J Thromb Haemost       Date:  2019-02-25       Impact factor: 5.824

10.  Imaging Subcellular Structures in the Living Zebrafish Embryo.

Authors:  Peter Engerer; Gabriela Plucinska; Rachel Thong; Laura Trovò; Dominik Paquet; Leanne Godinho
Journal:  J Vis Exp       Date:  2016-04-02       Impact factor: 1.355
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