Ablat Abliz1, Qimangul Aji1, Elzira Abdusalam1, Xiaowei Sun1, Adil Abdurahman1, Wenting Zhou1, Nicholas Moore2, Anwar Umar3. 1. Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China. 2. Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China; Department of Pharmacology, University Bordeaux Segalen, 146 rue Leo Saignat, 33076 Bordeaux, France. Electronic address: nicholas.moore@pharmaco.u-bordeaux2.fr. 3. Department of Pharmacology, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, PR China; Department of Pharmacology, University Bordeaux Segalen, 146 rue Leo Saignat, 33076 Bordeaux, France. Electronic address: anwar.umar@126.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Cydonia oblonga Mill. leaves are traditionally used in Uyghur medicine to treat or prevent cardiovascular disease. Beyond a demonstrated effect on thrombosis, we tested it for an effect on dyslipidemia, in a rat model of hyperlipidemia. METHODS: Seventy healthy Sprague Dawley rats were randomly divided into 6 groups: normal controls, model controls, simvastatin, and low-, medium- and high-dose Cydonia oblonga Mill. leaf extracts (COM), orally for 56 days. The normal controls were fed a normal diet, all other groups a high fat diet. Rat weights were recorded over time. Total cholesterol (TC), triglycerides (TG), low and high-density lipoproteins (LDL, HDL), as well as AST, ALT and total protein (TP) were measured in serum at the end of the study. The antioxidant capacity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), malondialdehyde (MDA) was measured in liver samples, along with lipoprotein lipase (LPL), and hepatic lipase (HL). Liver pathology was described. RESULTS: COM dose-dependently reduced TC, TG, LDL-C and MDA, inhibited the activity of ALT, AST and LPS, increased HDL-C content, increased the activity of SOD, GSH-PX, LPL and HL, and reduced liver steatosis in hyperlipidaemia rats, which was significant at medium and high doses. The effect of COM was similar to that of simvastatin except for increased LPL and HL which were reduced by COM but not by simvastatin. CONCLUSION: Cydonia oblonga Mill. leaf extracts have hypolipidaemic and hepatoprotective effects, probably related to increasing antioxidant capacity and lipoprotein metabolism in the liver, and inhibition of lipogenesis.
ETHNOPHARMACOLOGICAL RELEVANCE: Cydonia oblonga Mill. leaves are traditionally used in Uyghur medicine to treat or prevent cardiovascular disease. Beyond a demonstrated effect on thrombosis, we tested it for an effect on dyslipidemia, in a rat model of hyperlipidemia. METHODS: Seventy healthy Sprague Dawley rats were randomly divided into 6 groups: normal controls, model controls, simvastatin, and low-, medium- and high-dose Cydonia oblonga Mill. leaf extracts (COM), orally for 56 days. The normal controls were fed a normal diet, all other groups a high fat diet. Rat weights were recorded over time. Total cholesterol (TC), triglycerides (TG), low and high-density lipoproteins (LDL, HDL), as well as AST, ALT and total protein (TP) were measured in serum at the end of the study. The antioxidant capacity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), malondialdehyde (MDA) was measured in liver samples, along with lipoprotein lipase (LPL), and hepatic lipase (HL). Liver pathology was described. RESULTS: COM dose-dependently reduced TC, TG, LDL-C and MDA, inhibited the activity of ALT, AST and LPS, increased HDL-C content, increased the activity of SOD, GSH-PX, LPL and HL, and reduced liver steatosis in hyperlipidaemia rats, which was significant at medium and high doses. The effect of COM was similar to that of simvastatin except for increased LPL and HL which were reduced by COM but not by simvastatin. CONCLUSION:Cydonia oblonga Mill. leaf extracts have hypolipidaemic and hepatoprotective effects, probably related to increasing antioxidant capacity and lipoprotein metabolism in the liver, and inhibition of lipogenesis.
Authors: Abdulaziz M S AlSaad; Fawaz Alasmari; Hatem M Abuohashish; Mohamed Mohany; Mohammed M Ahmed; Salim S Al-Rejaie Journal: Redox Rep Date: 2020-12 Impact factor: 4.412