BACKGROUND: It is increasingly evident that CD8(+) T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8(+)CD25(+) T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8(+)CD25(+) T cells in experimental atherosclerosis were investigated in this study. METHODS AND RESULTS: CD8(+)CD25(+) T cells were observed in atherosclerotic plaques of apoE(-/-) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8(+)CD25(+) T cells from apoE(-/-) mice. Depletion of CD8(+)CD25(+) from total CD8(+) T cells rendered higher cytolytic activity of the remaining CD8(+)CD25(-) T cells. Adoptive transfer of CD8(+)CD25(+) T cells into apoE(-/-) mice suppressed the proliferation of splenic CD4(+) T cells and significantly reduced atherosclerosis in recipient mice. CONCLUSIONS: Our study has identified an athero-protective role for CD8(+)CD25(+) T cells in experimental atherosclerosis.
BACKGROUND: It is increasingly evident that CD8(+) T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8(+)CD25(+) T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8(+)CD25(+) T cells in experimental atherosclerosis were investigated in this study. METHODS AND RESULTS: CD8(+)CD25(+) T cells were observed in atherosclerotic plaques of apoE(-/-) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8(+)CD25(+) T cells from apoE(-/-) mice. Depletion of CD8(+)CD25(+) from total CD8(+) T cells rendered higher cytolytic activity of the remaining CD8(+)CD25(-) T cells. Adoptive transfer of CD8(+)CD25(+) T cells into apoE(-/-) mice suppressed the proliferation of splenic CD4(+) T cells and significantly reduced atherosclerosis in recipient mice. CONCLUSIONS: Our study has identified an athero-protective role for CD8(+)CD25(+) T cells in experimental atherosclerosis.
Authors: Peter M Mihailovic; Wai Man Lio; Juliana Yano; Xiaoning Zhao; Jianchang Zhou; Kuang-Yuh Chyu; Prediman K Shah; Bojan Cercek; Paul C Dimayuga Journal: PLoS One Date: 2017-11-01 Impact factor: 3.240
Authors: Paul C Dimayuga; Xiaoning Zhao; Juliana Yano; Wai Man Lio; Jianchang Zhou; Peter M Mihailovic; Bojan Cercek; Prediman K Shah; Kuang-Yuh Chyu Journal: J Am Heart Assoc Date: 2017-07-15 Impact factor: 5.501
Authors: Lindsey E Padgett; Huy Q Dinh; Runpei Wu; Dalia E Gaddis; Daniel J Araujo; Holger Winkels; Anh Nguyen; Coleen A McNamara; Catherine C Hedrick Journal: Arterioscler Thromb Vasc Biol Date: 2020-10-15 Impact factor: 10.514
Authors: Antonio Bilancio; Barbara Rinaldi; Maria Antonietta Oliviero; Maria Donniacuo; Maria Gaia Monti; Amedeo Boscaino; Irene Marino; Lori Friedman; Francesco Rossi; Bart Vanhaesebroeck; Antimo Migliaccio Journal: Biosci Rep Date: 2017-09-27 Impact factor: 3.840