Recep Eröz1, Kursad Unluhizarci2, Nurhan Cucer3, Figen Ozturk4. 1. Department of Medical Genetics, Duzce University Medical School, Duzce, Turkey. eroz38r@hotmail.com 2. Division of Endocrinology, Department of Internal Medicine, Erciyes University Medical School, Kayseri, Turkey. 3. Department of Medical Biology, Erciyes University Medical School, Kayseri, Turkey. 4. Department of Pathology, Erciyes University Medical School, Kayseri, Turkey.
Abstract
OBJECTIVE: To evaluate the diagnostic value of argyrophilic nucleolar organizing regions (AgNORs) in the nondiagnostic fine needle aspiration biopsy (NFNAB) specimens (due to insufficient cell groups) of cases with thyroid nodules. STUDY DESIGN: Thirty-five patients with NFNAB were included in the study. FNAB stained with Giemsa was faded using the McKee technique, then stained for AgNOR detection according to a specific protocol. One hundred nuclei per individual were evaluated to detect the AgNOR count/nucleus and total AgNOR area/ nuclear area (TNA/NA) of individual cells by using a computer program. RESULTS: The AgNOR values of patients with NFNAB were between 1.2-2.4% for AgNOR count/ nucleus and between 3.1-9.1%for TNA/NA. When the cutoff values were taken as > 3 for AgNOR count/nucleus and > 8 for TNA/NA, the sensitivity ratios were 100% and 97% for AgNOR count/nucleus and TNA/ NA for discriminating benign/malignant lesions. CONCLUSION: By using some cutoff values obtained from a modified AgNOR staining method, benign/malignant potential of thyroid aspirations with NFNAB may be estimated. (Anal Quant Cytopathol Histopathol
OBJECTIVE: To evaluate the diagnostic value of argyrophilic nucleolar organizing regions (AgNORs) in the nondiagnostic fine needle aspiration biopsy (NFNAB) specimens (due to insufficient cell groups) of cases with thyroid nodules. STUDY DESIGN: Thirty-five patients with NFNAB were included in the study. FNAB stained with Giemsa was faded using the McKee technique, then stained for AgNOR detection according to a specific protocol. One hundred nuclei per individual were evaluated to detect the AgNOR count/nucleus and total AgNOR area/ nuclear area (TNA/NA) of individual cells by using a computer program. RESULTS: The AgNOR values of patients with NFNAB were between 1.2-2.4% for AgNOR count/ nucleus and between 3.1-9.1%for TNA/NA. When the cutoff values were taken as > 3 for AgNOR count/nucleus and > 8 for TNA/NA, the sensitivity ratios were 100% and 97% for AgNOR count/nucleus and TNA/ NA for discriminating benign/malignant lesions. CONCLUSION: By using some cutoff values obtained from a modified AgNOR staining method, benign/malignant potential of thyroid aspirations with NFNAB may be estimated. (Anal Quant Cytopathol Histopathol
Authors: Dmitriy Sergeevich Kobyakov; Ashot Merudzhanovich Avdalyan; Aleksandr Fedorovich Lazarev; Elena Leonidovna Lushnikova; Lev Moiseevich Nepomnyashchikh Journal: Cancer Biol Med Date: 2014-12 Impact factor: 4.248