H Zhu1, H Cai, M Tang, J Tang. 1. Orthopaedics Department, Xuhui Central Hospital, No.966, Middle Huaihai Road, Shanghai, 200031, China.
Abstract
AIM: Neuropilin (NRP)-1, a co-receptor for vascular endothelial growth factor (VEGF), plays an important role in angiogenesis and malignant progression of many cancers. However, the involvement of NRP-1 in osteosarcoma is not completely understood. The aim of this study was to investigate the expression pattern and clinical significance of NRP-1 in human osteosarcoma. METHODS: NRP-1 mRNA and protein expression levels were detected by RT-PCR and Western blot assays, respectively, using 166 pairs of osteosarcoma and noncancerous bone tissues. Then, the association of NRP-1 expression with clinicopathological factors or survival of osteosarcoma patients was further evaluated. RESULTS: RT-PCR and Western blot assays revealed that NRP-1 expression in osteosarcoma tissues was significantly higher than that in corresponding noncancerous bone tissues at both mRNA and protein levels (both P < 0.001). In addition, high NRP-1 expression more frequently occurred in osteosarcoma tissues with advanced clinical stage (P = 0.006), positive distant metastasis (P = 0.01) and poor response to chemotherapy (P = 0.006). Moreover, osteosarcoma patients with high NRP-1 expression had significantly shorter overall survival and disease-free survival (both P < 0.001) when compared with patients with the low expression of NRP-1. On Cox multivariate analysis, NRP-1 overexpression was an independent and significant prognostic factor to predict poor overall survival and disease-free survival (both P = 0.001). CONCLUSION: This is the first study to reveal that NRP-1 overexpression may be related to the prediction of metastasis potency, response to chemotherapy and poor prognosis for osteosarcoma patients, suggesting that NRP-1 may serve as a prognostic marker for the optimization of clinical treatments.
AIM: Neuropilin (NRP)-1, a co-receptor for vascular endothelial growth factor (VEGF), plays an important role in angiogenesis and malignant progression of many cancers. However, the involvement of NRP-1 in osteosarcoma is not completely understood. The aim of this study was to investigate the expression pattern and clinical significance of NRP-1 in humanosteosarcoma. METHODS:NRP-1 mRNA and protein expression levels were detected by RT-PCR and Western blot assays, respectively, using 166 pairs of osteosarcoma and noncancerous bone tissues. Then, the association of NRP-1 expression with clinicopathological factors or survival of osteosarcomapatients was further evaluated. RESULTS: RT-PCR and Western blot assays revealed that NRP-1 expression in osteosarcoma tissues was significantly higher than that in corresponding noncancerous bone tissues at both mRNA and protein levels (both P < 0.001). In addition, high NRP-1 expression more frequently occurred in osteosarcoma tissues with advanced clinical stage (P = 0.006), positive distant metastasis (P = 0.01) and poor response to chemotherapy (P = 0.006). Moreover, osteosarcomapatients with high NRP-1 expression had significantly shorter overall survival and disease-free survival (both P < 0.001) when compared with patients with the low expression of NRP-1. On Cox multivariate analysis, NRP-1 overexpression was an independent and significant prognostic factor to predict poor overall survival and disease-free survival (both P = 0.001). CONCLUSION: This is the first study to reveal that NRP-1 overexpression may be related to the prediction of metastasis potency, response to chemotherapy and poor prognosis for osteosarcomapatients, suggesting that NRP-1 may serve as a prognostic marker for the optimization of clinical treatments.
Authors: Adrian M Jubb; Laura A Strickland; Scot D Liu; Judy Mak; Maike Schmidt; Hartmut Koeppen Journal: J Pathol Date: 2011-10-25 Impact factor: 7.996
Authors: Jun Ah Lee; Min Suk Kim; Dong Ho Kim; Jung Sub Lim; Ji Young Yoo; Jae Soo Koh; Soo-Yong Lee; Dae-Geun Jeon; Kyung Duk Park Journal: Pediatr Blood Cancer Date: 2008-02 Impact factor: 3.167
Authors: Anthony Lanahan; Xi Zhang; Alessandro Fantin; Zhen Zhuang; Felix Rivera-Molina; Katherine Speichinger; Claudia Prahst; Jiasheng Zhang; Yingdi Wang; George Davis; Derek Toomre; Christiana Ruhrberg; Michael Simons Journal: Dev Cell Date: 2013-04-29 Impact factor: 12.270
Authors: Aleksandar Boro; Matthias Je Arlt; Harald Lengnick; Bernhard Robl; Maren Husmann; Josefine Bertz; Walter Born; Bruno Fuchs Journal: Am J Transl Res Date: 2015-03-15 Impact factor: 4.060
Authors: Navatha Shree Polavaram; Samikshan Dutta; Ridwan Islam; Arup K Bag; Sohini Roy; David Poitz; Jeffrey Karnes; Lorenz C Hofbauer; Manish Kohli; Brian A Costello; Raffael Jimenez; Surinder K Batra; Benjamin A Teply; Michael H Muders; Kaustubh Datta Journal: Bone Res Date: 2021-05-14 Impact factor: 13.567
Authors: Laura Lorés-Motta; Freekje van Asten; Philipp S Muether; Dzenita Smailhodzic; Joannes M Groenewoud; Amer Omar; John Chen; Robert K Koenekoop; Sascha Fauser; Carel B Hoyng; Anneke I den Hollander; Eiko K de Jong Journal: Pharmacogenet Genomics Date: 2016-01 Impact factor: 2.089
Authors: Sohini Roy; Arup K Bag; Rakesh K Singh; James E Talmadge; Surinder K Batra; Kaustubh Datta Journal: Front Immunol Date: 2017-10-10 Impact factor: 7.561