| Literature DB >> 24338125 |
Mohammad Hassanzadeh Saber1, Shahrouz Baradaran Noveiri, Mohammad Pourkazemi, Mohammadali Yazdani, Ahmad Ghoroghi, Mahmoud Bahmani, Mohammad Pourdehghani, Fereidoon Chakmehdouz, Mahtab Yarmohammadi, Mohammadreza Nowruzfashkhami.
Abstract
Diploid gynogenesis was induced in ship sturgeon Acipenser nudiventris using UV-irradiated sperm from Siberian sturgeon Acipenser baerii. The optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. The temperature of cold shock and its duration were 2.5 °C and 30 min, respectively. A total of 30 gynogens of known parentage from experimental treatments were screened using microsatellite DNA analysis, and uniparental transmission in meiogens was confirmed. The results show that heterologous Siberian sturgeon sperm is applicable as UV-irradiated sperm for the induction of gynogenesis in ship sturgeon. This technique may recover the critically endangered sturgeon species that are becoming extinct.Entities:
Mesh：
Year: 2013 PMID： 24338125 DOI： 10.1007/s13353-013-0188-3
Source DB: PubMed Journal: J Appl Genet ISSN： 1234-1983 Impact factor: 3.240