Literature DB >> 2433768

Epitope mapping by chemical modification of free and antibody-bound protein antigen.

A Burnens, S Demotz, G Corradin, H Binz, H R Bosshard.   

Abstract

A monoclonal antibody bound to a protein antigen slows the rate of chemical modification of amino acid residues located at the epitope. By comparing the degree of acetylation of 18 lysine and 7 threonine residues in free and antibody-bound horse cytochrome c, a discontiguous, conformational epitope was characterized on this protein antigen. The new approach is particularly suitable to probe discontiguous and conformational epitopes, which are difficult to analyze by other procedures.

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Year:  1987        PMID： 2433768     DOI： 10.1126/science.2433768

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  13 in total

1.  Characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) core protein p24 by epitope excision and differential chemical modification followed by mass spectrometric peptide mapping analysis.

Authors:  E O Hochleitner; C Borchers; C Parker; R J Bienstock; K B Tomer
Journal:  Protein Sci       Date:  2000-03       Impact factor: 6.725

2.  MALDI/MS-based epitope mapping of antigens bound to immobilized antibodies.

Authors:  Carol E Parker; Kenneth B Tomer
Journal:  Mol Biotechnol       Date:  2002-01       Impact factor: 2.695

3.  Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin.

Authors:  S G Sedgwick; T M Nguyen; J M Ellis; H Crowne; G E Morris
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

4.  Mass spectrometric study of the effects of hydrophobic surface chemistry and morphology on the digestion of surface-bound proteins.

Authors:  Alan Doucette; David Craft; Liang Li
Journal:  J Am Soc Mass Spectrom       Date:  2003-03       Impact factor: 3.109

5.  Monoclonal antibody studies of creatine kinase. Antibody-binding sites in the N-terminal region of creatine kinase and effects of antibody on enzyme refolding.

Authors:  G E Morris; L C Frost; P A Newport; N Hudson
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

6.  Epitope mapping of recombinant antigens by transposon mutagenesis.

Authors:  G E Morris; S G Sedgwick
Journal:  Mol Biotechnol       Date:  1995-08       Impact factor: 2.695

7.  Role of Myxococcus xanthus cell surface antigen 1604 in development.

Authors:  B W Jarvis; M Dworkin
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

8.  An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR.

Authors:  Y Paterson; S W Englander; H Roder
Journal:  Science       Date:  1990-08-17       Impact factor: 47.728

9.  A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants.

Authors:  K Tomizawa; E Mine; A Fujii; Y Y Ohashi; S Yamagoe; Y Hashimoto; A Ishida-Okawara; M Ito; M Tanokura; T Yamamoto; Y Arimura; T Nagasawa; S Mizuno; K Suzuki
Journal:  J Clin Immunol       Date:  1998-03       Impact factor: 8.317

10.  Binding of ferredoxin to ferredoxin:NADP+ oxidoreductase: the role of carboxyl groups, electrostatic surface potential, and molecular dipole moment.

Authors:  A R De Pascalis; I Jelesarov; F Ackermann; W H Koppenol; M Hirasawa; D B Knaff; H R Bosshard
Journal:  Protein Sci       Date:  1993-07       Impact factor: 6.725
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