PURPOSE: To measure high-resolution eye-specific displacements and deformations induced within the human LC microstructure by an acute increase in IOP. METHODS: Six eyes from donors aged 23 to 82 were scanned using second harmonic-generated (SHG) imaging at various levels of IOP from 10 to 50 mm Hg. An image registration technique was developed, tested, and used to find the deformation mapping between maximum intensity projection images acquired at low and elevated IOP. The mappings were analyzed to determine the magnitude and distribution of the IOP-induced displacements and deformations and contralateral similarity. RESULTS: Images of the LC were obtained and the registration technique was successful. IOP increases produced substantial, and potentially biologically significant, levels of in-plane LC stretch and compression (reaching 10%-25% medians and 20%-30% 75th percentiles). Deformations were sometimes highly focal and concentrated in regions as small as a few pores. Regions of largest displacement, stretch, compression, and shear did not colocalize. Displacements and strains were not normally distributed. Contralateral eyes did not always have more similar responses to IOP than unrelated eyes. Under elevated IOP, some LC regions were under bi-axial stretch, others under bi-axial compression. CONCLUSIONS: We obtained eye-specific measurements of the complex effects of IOP on the LC with unprecedented resolution in uncut and unfixed human eyes. Our technique was robust to electronic and speckle noise. Elevated IOP produced substantial in-plane LC stretch and compression. Further research will explore the effects of IOP on the LC in a three-dimensional framework.
PURPOSE: To measure high-resolution eye-specific displacements and deformations induced within the human LC microstructure by an acute increase in IOP. METHODS: Six eyes from donors aged 23 to 82 were scanned using second harmonic-generated (SHG) imaging at various levels of IOP from 10 to 50 mm Hg. An image registration technique was developed, tested, and used to find the deformation mapping between maximum intensity projection images acquired at low and elevated IOP. The mappings were analyzed to determine the magnitude and distribution of the IOP-induced displacements and deformations and contralateral similarity. RESULTS: Images of the LC were obtained and the registration technique was successful. IOP increases produced substantial, and potentially biologically significant, levels of in-plane LC stretch and compression (reaching 10%-25% medians and 20%-30% 75th percentiles). Deformations were sometimes highly focal and concentrated in regions as small as a few pores. Regions of largest displacement, stretch, compression, and shear did not colocalize. Displacements and strains were not normally distributed. Contralateral eyes did not always have more similar responses to IOP than unrelated eyes. Under elevated IOP, some LC regions were under bi-axial stretch, others under bi-axial compression. CONCLUSIONS: We obtained eye-specific measurements of the complex effects of IOP on the LC with unprecedented resolution in uncut and unfixed human eyes. Our technique was robust to electronic and speckle noise. Elevated IOP produced substantial in-plane LC stretch and compression. Further research will explore the effects of IOP on the LC in a three-dimensional framework.
Authors: Michael D Roberts; Ian A Sigal; Yi Liang; Claude F Burgoyne; J Crawford Downs Journal: Invest Ophthalmol Vis Sci Date: 2010-06-10 Impact factor: 4.799
Authors: Michaël J A Girard; Nicholas G Strouthidis; Adrien Desjardins; Jean Martial Mari; C Ross Ethier Journal: J R Soc Interface Date: 2013-07-24 Impact factor: 4.118
Authors: Anthony J Bellezza; Christopher J Rintalan; Hilary W Thompson; J Crawford Downs; Richard T Hart; Claude F Burgoyne Journal: Invest Ophthalmol Vis Sci Date: 2003-02 Impact factor: 4.799
Authors: Ian A Sigal; John G Flanagan; Kira L Lathrop; Inka Tertinegg; Richard Bilonick Journal: Invest Ophthalmol Vis Sci Date: 2012-10-03 Impact factor: 4.799
Authors: Ning-Jiun Jan; Jonathan L Grimm; Huong Tran; Kira L Lathrop; Gadi Wollstein; Richard A Bilonick; Hiroshi Ishikawa; Larry Kagemann; Joel S Schuman; Ian A Sigal Journal: Biomed Opt Express Date: 2015-11-05 Impact factor: 3.732
Authors: Howard Lockwood; Juan Reynaud; Stuart Gardiner; Jonathan Grimm; Vincent Libertiaux; J Crawford Downs; Hongli Yang; Claude F Burgoyne Journal: Invest Ophthalmol Vis Sci Date: 2015-02-03 Impact factor: 4.799
Authors: M Gómez-Mariscal; B Puerto; F J Muñoz-Negrete; V de Juan; G Rebolleda Journal: Graefes Arch Clin Exp Ophthalmol Date: 2019-06-28 Impact factor: 3.117
Authors: Yik Tung Tracy Ling; Ran Shi; Dan E Midgett; Joan L Jefferys; Harry A Quigley; Thao D Nguyen Journal: Invest Ophthalmol Vis Sci Date: 2019-06-03 Impact factor: 4.799
Authors: Leon C Ho; Ian A Sigal; Ning-Jiun Jan; Alexander Squires; Zion Tse; Ed X Wu; Seong-Gi Kim; Joel S Schuman; Kevin C Chan Journal: Invest Ophthalmol Vis Sci Date: 2014-08-07 Impact factor: 4.799