| Literature DB >> 24333583 |
Ju-Young Ko1, Eun-A Kim1, Ji-Hyeok Lee1, Min-Cheol Kang1, Jung-Suck Lee2, Jin-Soo Kim3, Won-Kyo Jung4, You-Jin Jeon5.
Abstract
This study investigates the protective effect of aquacultured flounder fish-derived peptide (AFFP) against 2,2-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in a zebrafish model. Zebrafish embryos were evaluated for the protective effect by heartbeat rate, survival rate, ROS generation, lipid peroxidation, and cell death. In the results, the AAPH group showed a low survival rate, whereas the AFFP and AAPH co-treated group increased a survival rate. Also, AFFP dose-dependently reduced AAPH-induced intracellular ROS and lipid peroxidation, and decreased cell death in AAPH-induced zebrafish. These results revealed that AFFP could be used as a natural antioxidant, and that the zebrafish provides an alternative in vivo model to efficiently evaluate the antioxidative effects of peptides on fishes.Entities:
Keywords: AAPH; Antioxidative peptide; In vivo model; Oxidative stress; Zebrafish
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Year: 2013 PMID： 24333583 DOI： 10.1016/j.fsi.2013.11.018
Source DB: PubMed Journal: Fish Shellfish Immunol ISSN： 1050-4648 Impact factor: 4.581