| Literature DB >> 24333563 |
Syamantak Majumder1, Swaraj Sinha1, Jamila H Siamwala1, Ajit Muley1, Himabindu Reddy Seerapu1, Gopi Krishna Kolluru1, Vimal Veeriah1, Shunmugam Nagarajan1, Sree Rama Chaitanya Sridhara1, Mani Krishna Priya1, Maniselvan Kuppusamy1, Sundaramoorthy Srinivasan1, Salini Konikkat1, Gowrishankar Soundararajan1, S Venkataraman2, Uttara Saran1, Suvro Chatterjee3.
Abstract
Nitric oxide (NO) is a known modulator of angiogenesis. The NONOate subfamily of NO donors has long been used in experimental and clinical studies to promote angiogenesis. However, no studies have been conducted yet to compare the angiogenesis potential of these NO donors in respect to their pattern of NO release. We hypothesize that having different pattern of NO release, each of the NO donors in NONOate subfamily can promote key stages of angiogenesis in differential manner. To verify our hypothesis, NO donors with half life ranging from seconds to several hours and having very different pattern of NO release were selected to evaluate their efficacy in modulating angiogenesis. Endothelial tube formation using EAhy926 cells was maximally increased by Spermine NONOate (SP) treatment. SP treatment maximally induced both ex vivo and in vivo angiogenesis using egg yolk and cotton plug angiogenesis models respectively. Experiment using chick embryo partial ischemia model revealed SP as the best suited NO donor to recover ischemia driven hampered angiogenesis. The present study elaborated that differential release pattern of NO by different NO donors can modulate angiogenesis differentially and also suggested that SP have a unique pattern of NO release that best fits for angiogenesis.Entities:
Keywords: Angiogenesis; Nitric oxide; Nitric oxide donors; cGMP
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Year: 2013 PMID： 24333563 DOI： 10.1016/j.niox.2013.12.002
Source DB: PubMed Journal: Nitric Oxide ISSN： 1089-8603 Impact factor: 4.427