Gyoseon Goya M Yeom1, Seona Min2, Sun Yeou Kim3. 1. Skin Science, R&D Center, iPEERES Cosmetics Ltd., 104, Je2gongdan 1-gil, Miyang-myeon, Anseong-si, Gyeonggi-do 456-843, Republic of Korea. Electronic address: goya@khu.ac.kr. 2. Laboratory of Microbiology, INOMAX, 16, Goji 5-gil, Jeongnam-myeon, Hwaseong-si, Gyeonggi-do, 445-962, Republic of Korea. Electronic address: inochall@naver.com. 3. Gachon Univ. of Medicine and Science, 191, Hambangmoe-ro, Yeonsu-gu, Incheon, 406-799 Korea. Electronic address: sunnykim@gachon.ac.kr.
Abstract
BACKGROUND: 2,3,5,6-Tetramethylpyrazine (TMP) is known as a composition of Ephedra sinica and it has been used in the treatment of several disorders such as asthma, heart failure, rhinitis, and urinary incontinence. It has been reported that TMP inhibits melanoma metastasis and suppression angiogenesis by VEGF. OBJECTIVE: The inhibitory activity of melanogenic proteins by TMP was confirmed in UVA-induced melanoma/keratinocyte co-culture system in this paper. METHODS: The melanin content, cell viability and cytokines release such as TNFα, IL-1β, IL-8 and GM-CSF were measured by ELISA assay. In addition, TRP1, MITF and MAPK signaling protein expression were also evaluated by Western blotting analysis. RESULTS: Decreasing melanogenic factors (TRP1, MITF, and MAPK) and factors (TNFα, IL-1β, IL-8, and GM-CSF) improving skin cancer and inflammation were identified. CONCLUSION: It suggests that TMP can serve as a potent candidate for regulation of melanogenesis.
BACKGROUND:2,3,5,6-Tetramethylpyrazine (TMP) is known as a composition of Ephedra sinica and it has been used in the treatment of several disorders such as asthma, heart failure, rhinitis, and urinary incontinence. It has been reported that TMP inhibits melanoma metastasis and suppression angiogenesis by VEGF. OBJECTIVE: The inhibitory activity of melanogenic proteins by TMP was confirmed in UVA-induced melanoma/keratinocyte co-culture system in this paper. METHODS: The melanin content, cell viability and cytokines release such as TNFα, IL-1β, IL-8 and GM-CSF were measured by ELISA assay. In addition, TRP1, MITF and MAPK signaling protein expression were also evaluated by Western blotting analysis. RESULTS: Decreasing melanogenic factors (TRP1, MITF, and MAPK) and factors (TNFα, IL-1β, IL-8, and GM-CSF) improving skin cancer and inflammation were identified. CONCLUSION: It suggests that TMP can serve as a potent candidate for regulation of melanogenesis.
Authors: Hyo In Kim; Se Hyang Hong; Jin Mo Ku; Sooyeon Kang; Tai Young Kim; Yong Cheol Shin; Seong-Gyu Ko Journal: BMC Complement Altern Med Date: 2017-03-31 Impact factor: 3.659