Guodong Zhu1, Liang Liang1, Lei Li2, Qiang Dang1, Wenbin Song1, Shuyuan Yeh3, Dalin He4, Chawnshang Chang5. 1. Sex Hormone Research Center, Department of Urology, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China; George H. Whipple Lab for Cancer Research, Departments of Pathology and Urology, Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY. 2. Sex Hormone Research Center, Department of Urology, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China; George H. Whipple Lab for Cancer Research, Departments of Pathology and Urology, Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY. Electronic address: lilydr@hotmail.com. 3. George H. Whipple Lab for Cancer Research, Departments of Pathology and Urology, Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY. 4. Sex Hormone Research Center, Department of Urology, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China. Electronic address: hedlxjtu@yahoo.com.cn. 5. George H. Whipple Lab for Cancer Research, Departments of Pathology and Urology, Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY; Sex Hormone Research Center, China Medical University/Hospital, Taichung, Taiwan.
Abstract
OBJECTIVE: To investigate the expression of androgen receptor (AR) with clinical and pathologic features in patients with renal cell carcinoma (RCC) and to explore the function of AR using human RCC cells. MATERIALS AND METHODS: The expression of AR was detected by immunohistochemistry in 44 adjacent normal kidney tissues of 120 RCC patients and also in 16 metastatic RCC patients with their respective primary and metastatic tissue samples. The expression of AR was examined by western blot in commonly used human RCC cell lines and normal kidney epithelial cells, and the luciferase assay was performed in those AR-positive RCC cells. RESULTS: The expression rate of AR was higher in adjacent normal kidney (90.9%) than in RCC tissues (30.0%, P <.001), and it was negatively associated with pT stage and Fuhrman's grade. Specifically, there were 40.7% AR-positive cases in pT1 compared with 8.0% in pT3 (P = .013), and 50.0% of grade I cases were found to be AR positive compared with 12.9% in grade III (P = .008). AR expression was slightly higher in primary RCC tissues (12.5%) than their respective metastases (0%, P = .484). AR strongly expressed in CAKI-2 and OSRC-2 cells with little transactivation, which might indicate that AR in those 2 RCC cells has little function. CONCLUSION: Our results suggest that any attempt to investigate the roles of AR in RCC progression might need to combine the detection of AR expression in tissue samples with examining its function to make a correct correlation between AR and RCC progression.
OBJECTIVE: To investigate the expression of androgen receptor (AR) with clinical and pathologic features in patients with renal cell carcinoma (RCC) and to explore the function of AR using humanRCC cells. MATERIALS AND METHODS: The expression of AR was detected by immunohistochemistry in 44 adjacent normal kidney tissues of 120 RCCpatients and also in 16 metastatic RCCpatients with their respective primary and metastatic tissue samples. The expression of AR was examined by western blot in commonly used humanRCC cell lines and normal kidney epithelial cells, and the luciferase assay was performed in those AR-positive RCC cells. RESULTS: The expression rate of AR was higher in adjacent normal kidney (90.9%) than in RCC tissues (30.0%, P <.001), and it was negatively associated with pT stage and Fuhrman's grade. Specifically, there were 40.7% AR-positive cases in pT1 compared with 8.0% in pT3 (P = .013), and 50.0% of grade I cases were found to be AR positive compared with 12.9% in grade III (P = .008). AR expression was slightly higher in primary RCC tissues (12.5%) than their respective metastases (0%, P = .484). AR strongly expressed in CAKI-2 and OSRC-2 cells with little transactivation, which might indicate that AR in those 2 RCC cells has little function. CONCLUSION: Our results suggest that any attempt to investigate the roles of AR in RCC progression might need to combine the detection of AR expression in tissue samples with examining its function to make a correct correlation between AR and RCC progression.
Authors: Remi Adelaiye-Ogala; Nur P Damayanti; Ashley R Orillion; Sreevani Arisa; Sreenivasulu Chintala; Mark A Titus; Chinghai Kao; Roberto Pili Journal: Cancer Res Date: 2018-03-23 Impact factor: 12.701
Authors: Rebeca Osca-Verdegal; Jesús Beltrán-García; José Luis Górriz; José María Martínez Jabaloyas; Federico V Pallardó; José Luis García-Giménez Journal: Front Cell Dev Biol Date: 2022-06-22
Authors: Elizabeth M Williams; John P Higgins; Ankur R Sangoi; Jesse K McKenney; Megan L Troxell Journal: Int Urol Nephrol Date: 2014-09-14 Impact factor: 2.370