Literature DB >> 2433191

RNA colony hybridization method.

I Ivanov, L Gigova.   

Abstract

A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.

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Year:  1986        PMID： 2433191     DOI： 10.1016/0378-1119(86)90413-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  5 in total

1.  RNA colony blot hybridization method for enumeration of culturable Vibrio cholerae and Vibrio mimicus bacteria.

Authors:  Christopher J Grim; Young-Gun Zo; Nur A Hasan; Afsar Ali; Wasimul B Chowdhury; Atiqul Islam; Mohammed H Rashid; Munirul Alam; J Glenn Morris; Anwar Huq; Rita R Colwell
Journal:  Appl Environ Microbiol       Date:  2009-06-26       Impact factor: 4.792

2.  Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe.

Authors:  A S Rehnstam; A Norqvist; H Wolf-Watz; A Hagström
Journal:  Appl Environ Microbiol       Date:  1989-08       Impact factor: 4.792

3.  Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis.

Authors:  M Forsman; G Sandström; B Jaurin
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

4.  Characterization and classification of strains of Francisella tularensis isolated in the central Asian focus of the Soviet Union and in Japan.

Authors:  G Sandström; A Sjöstedt; M Forsman; N V Pavlovich; B N Mishankin
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

5.  Rapid detection and identification of Vibrio anguillarum by using a specific oligonucleotide probe complementary to 16S rRNA.

Authors:  J Martínez-Picado; A R Blanch; J Jofre
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792
  5 in total

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