Roosmarijn Luttmer1, Annemieke M Spijkerman2, Robert M Kok3, Carel Jakobs3, Henk J Blom3, Erik H Serne4, Jacqueline M Dekker5, Yvo M Smulders6. 1. Faculty of Medicine, VU University Medical Center, Amsterdam, The Netherlands. 2. Center for Prevention and Health Services Research, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. 3. Department of Clinical Chemistry and Institute for Cardiovascular Research ICaR-VU, VU University Medical Center, Amsterdam, The Netherlands. 4. Department of Internal Medicine and Institute for Cardiovascular Research ICaR-VU, VU University Medical Center, The Netherlands. 5. Institute for Research in Extramural Medicine (EMGO Institute), VU University Medical Centre, Amsterdam, The Netherlands. 6. Department of Internal Medicine and Institute for Cardiovascular Research ICaR-VU, VU University Medical Center, The Netherlands. Electronic address:y.smulders@vumc.nl.
Abstract
BACKGROUND: Disturbances of DNA methylation have been associated with multiple diseases, including cardiovascular disease, cancer and, as some have suggested, glucometabolic disturbances. Our aim was to assess the association of the metabolic syndrome and its individual components with DNA methylation in a population-based study. MATERIALS AND METHODS: In a human population (n = 738) stratified by age, sex and glucose metabolism, we explored associations of the metabolic syndrome according to National Cholesterol Education Program/Adult Treatment Panel-III criteria and its individual components (fasting glucose, high-density lipoprotein cholesterol, triglycerides, blood pressure, waist circumference) with global leukocyte DNA methylation. DNA methylation was measured as the methylcytosine/cytosine ratio in peripheral leukocytes using liquid chromatography-tandem mass spectrometry. RESULTS: Individuals with the metabolic syndrome had relative DNA hypomethylation compared to participants without the syndrome (β = -0.05; p = 0.01). This association was mainly attributable to linear associations of two metabolic syndrome components with DNA methylation: fasting plasma glucose (β = -0.02; p = 0.004) and high-density lipoprotein cholesterol (β = 0.07; p = 0.004). People with type 2 diabetes or impaired glucose metabolism had DNA hypomethylation compared to normoglycemic individuals (β = -0.05; p = 0.004). CONCLUSIONS: DNA hypomethylation is independently associated with hyperglycemia and low high-density lipoprotein cholesterol, both essential components of the metabolic syndrome. The potential implications and direction of possible causality require further study. Â
BACKGROUND: Disturbances of DNA methylation have been associated with multiple diseases, including cardiovascular disease, cancer and, as some have suggested, glucometabolic disturbances. Our aim was to assess the association of the metabolic syndrome and its individual components with DNA methylation in a population-based study. MATERIALS AND METHODS: In a human population (n = 738) stratified by age, sex and glucose metabolism, we explored associations of the metabolic syndrome according to National Cholesterol Education Program/Adult Treatment Panel-III criteria and its individual components (fasting glucose, high-density lipoprotein cholesterol, triglycerides, blood pressure, waist circumference) with global leukocyte DNA methylation. DNA methylation was measured as the methylcytosine/cytosine ratio in peripheral leukocytes using liquid chromatography-tandem mass spectrometry. RESULTS: Individuals with the metabolic syndrome had relative DNA hypomethylation compared to participants without the syndrome (β = -0.05; p = 0.01). This association was mainly attributable to linear associations of two metabolic syndrome components with DNA methylation: fasting plasma glucose (β = -0.02; p = 0.004) and high-density lipoprotein cholesterol (β = 0.07; p = 0.004). People with type 2 diabetes or impaired glucose metabolism had DNA hypomethylation compared to normoglycemic individuals (β = -0.05; p = 0.004). CONCLUSIONS: DNA hypomethylation is independently associated with hyperglycemia and low high-density lipoprotein cholesterol, both essential components of the metabolic syndrome. The potential implications and direction of possible causality require further study. Â
Authors: Valentina Gonzalez-Jaramillo; Eliana Portilla-Fernandez; Marija Glisic; Trudy Voortman; Wichor Bramer; Rajiv Chowdhury; Anton J M Roks; A H Jan Danser; Taulant Muka; Jana Nano; Oscar H Franco Journal: J Hum Hypertens Date: 2019-07-25 Impact factor: 3.012
Authors: Mithun Das; Jin Sha; Bertha Hidalgo; Stella Aslibekyan; Anh N Do; Degui Zhi; Dianjianyi Sun; Tao Zhang; Shengxu Li; Wei Chen; Sathanur R Srinivasan; Hemant K Tiwari; Devin Absher; Jose M Ordovas; Gerald S Berenson; Donna K Arnett; Marguerite R Irvin Journal: PLoS One Date: 2016-01-25 Impact factor: 3.240