Faisel Khan1, Wen Ling Choong, Qingyou Du, Aleksandar Jovanovi'c. 1. Medical Research Institute, Division of Cardiovascular and Diabetic Medicine, Ninewells Hospital & Medical School, University of Dundee, Dundee, United Kingdom.
Abstract
PURPOSE: To date, there is a wide range of methods in use to assess endothelial function, each with its own advantages and limitations. Here, we tested hypothesis that real-time RT-PCR threshold value (Ct), which is reflective of mRNA level, for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from whole blood is indicative of endothelial function in humans. MATERIALS AND METHODS: To assess vascular function, we measured baseline skin perfusion, postocclusion reactive hyperemia (PORH), and brachial artery flow-mediated dilatation (FMD) and tested for a possible correlation between vascular responses and blood GAPDH real-time RT-PCR Ct value in 75 healthy volunteers. RESULTS: Tests known to measure, at least in part, endothelial function such as baseline skin perfusion, the 2-minute recovery PORH, and FMD exhibited significant positive correlations with blood GAPDH Ct values. In contrast, there was no significant correlation between Ct values for blood GAPDH and peak PORH, an endothelium-independent parameter. CONCLUSIONS: Based on these findings, we report that GAPDH mRNA level in the blood correlates with vascular function in healthy subjects. This suggests that GAPDH mRNA level could be a potential biomarker of vascular endothelial function.
PURPOSE: To date, there is a wide range of methods in use to assess endothelial function, each with its own advantages and limitations. Here, we tested hypothesis that real-time RT-PCR threshold value (Ct), which is reflective of mRNA level, for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from whole blood is indicative of endothelial function in humans. MATERIALS AND METHODS: To assess vascular function, we measured baseline skin perfusion, postocclusion reactive hyperemia (PORH), and brachial artery flow-mediated dilatation (FMD) and tested for a possible correlation between vascular responses and blood GAPDH real-time RT-PCR Ct value in 75 healthy volunteers. RESULTS: Tests known to measure, at least in part, endothelial function such as baseline skin perfusion, the 2-minute recovery PORH, and FMD exhibited significant positive correlations with blood GAPDH Ct values. In contrast, there was no significant correlation between Ct values for blood GAPDH and peak PORH, an endothelium-independent parameter. CONCLUSIONS: Based on these findings, we report that GAPDH mRNA level in the blood correlates with vascular function in healthy subjects. This suggests that GAPDH mRNA level could be a potential biomarker of vascular endothelial function.
Authors: Mary C Corretti; Todd J Anderson; Emelia J Benjamin; David Celermajer; Francois Charbonneau; Mark A Creager; John Deanfield; Helmut Drexler; Marie Gerhard-Herman; David Herrington; Patrick Vallance; Joseph Vita; Robert Vogel Journal: J Am Coll Cardiol Date: 2002-01-16 Impact factor: 24.094
Authors: Qingyou Du; Sofija Jovanović; Andriy Sukhodub; Evelyn Barratt; Ellen Drew; Katherine M Whalley; Vanessa Kay; Marie McLaughlin; Evelyn E Telfer; Christopher L R Barratt; Aleksandar Jovanović Journal: Hum Reprod Date: 2010-09-16 Impact factor: 6.918
Authors: Nilkantha Sen; Makoto R Hara; Michael D Kornberg; Matthew B Cascio; Byoung-Il Bae; Neelam Shahani; Bobby Thomas; Ted M Dawson; Valina L Dawson; Solomon H Snyder; Akira Sawa Journal: Nat Cell Biol Date: 2008-06-15 Impact factor: 28.824
Authors: Sofija Jovanović; Qingyou Du; Somnath Mukhopadhyay; Robert Swingler; Richard Buckley; Jane McEachen; Aleksandar Jovanović Journal: Clin Transl Sci Date: 2008-05 Impact factor: 4.689
Authors: Luana Xavier Soares Gomes Moura Fé; Eliane Pereira Cipolatti; Martina Costa Cerqueira Pinto; Suema Branco; Fábio César Sousa Nogueira; Gisela Maria Dellamora Ortiz; Anderson de Sá Pinheiro; Evelin Andrade Manoel Journal: Med Res Rev Date: 2022-06-28 Impact factor: 12.388