Literature DB >> 2432894

Point-mutated p21ras couples a muscarinic receptor to calcium channels and polyphosphoinositide hydrolysis.

V P Chiarugi, F Pasquali, S Vannucchi, M Ruggiero.   

Abstract

A high-affinity muscarinic receptor is detectable both in normal 3T3 mouse fibroblasts and in their transformed counterpart obtained by transfection with the oncogene EJ/T24-H-ras. However, only the transformed cell line is responsive to muscarinic agonist carbamylcholine in terms of Ca2+ influx and polyphosphoinositide hydrolysis, whereas the normal cell line is unresponsive. Using a point-mutated p21ras protein and monoclonal antibodies anti-p21ras, we provide evidences that p21ras couples to receptor-operating calcium channels and to polyphosphoinositide hydrolysis a muscarinic receptor which is uncoupled in normal mouse fibroblasts.

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Year:  1986        PMID： 2432894     DOI： 10.1016/s0006-291x(86)80214-5

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  p21ras-induced responsiveness of phosphatidylinositol turnover to bradykinin is a receptor number effect.

Authors:  J Downward; J de Gunzburg; R Riehl; R A Weinberg
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

2.  NIH-3T3 cells transformed with a ras oncogene exhibit a protein kinase C-mediated inhibition of agonist-stimulated Ca2+ inflow.

Authors:  A J Polverino; B P Hughes; G J Barritt
Journal:  Biochem J       Date:  1990-10-15       Impact factor: 3.857

3.  Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists.

Authors:  M T Diaz-Meco; P Larrodera; M Lopez-Barahona; M E Cornet; P G Barreno; J Moscat
Journal:  Biochem J       Date:  1989-10-01       Impact factor: 3.857

Review 4.  The structure and mechanism of neurotransmitter receptors. Implications for the structure and function of the central nervous system.

Authors:  P G Strange
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

5.  Desensitization of prostaglandin F2 alpha-stimulated inositol phosphate generation in NIH-3T3 fibroblasts transformed by overexpression of normal c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes.

Authors:  F M Black; M J Wakelam
Journal:  Biochem J       Date:  1990-05-01       Impact factor: 3.857

Review 6.  Arachidonic acid mobilization in platelets: the possible role of protein kinase C and G-proteins.

Authors:  M B Feinstein; S P Halenda
Journal:  Experientia       Date:  1988-02-15

7.  Transformation-specific decrease of phosphorylation of 80K protein, a substrate of protein kinase C, in NIH3T3 cells.

Authors:  M Oh-uchida; K Yano; S Kawamoto; K Shimizu
Journal:  Jpn J Cancer Res       Date:  1990-08
  7 in total

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