Two new furostanol saponins from the fibrous root of Ophiopogon japonicus.

Two new furostanol saponins ophiopogonins J (1) and K (2) were isolated from the fibrous roots of Ophiopogon japonicus. The structures of 1 and 2 were established as (25R)-26-O-[(β-D-glucopyranosyl-(1 --> 2)-β-D-glucopyranosyl)]-14-hydroxy-furost-5,20(22)-diene 3-O-[α-L-rhamnopyranosyl-(1 --> 2)]-β-D-glucopyranoside (1), and (25R)-26-O-[(β-D-glucopyranosyl-(1 --> 2)-β-D-glucopyranosyl)]-furost-5,20(22)-diene 3-O-α-L-rhamnopyranosyl-(1 --> 2)[(β-D-xylopyranosyl-(1 --> 4)-β-D-glucopyranoside)] (2) on the basis of spectroscopic means including HRESIMS, 1D, and 2D NMR experiments.