| Literature DB >> 24326267 |
Claudia Baumann1, John O'Rourke1, Gemma Swiers1, Eleni Giannoulatou2, Stephen Taylor2, Anagha Joshi3, Victoria Moignard3, Cristina Pina1, Thomas Bee1, Konstantinos D Kokkaliaris4, Momoko Yoshimoto5, Mervin C Yoder5, Jon Frampton6, Timm Schroeder4, Tariq Enver1, Berthold Göttgens3, Marella F T R de Bruijn1.
Abstract
Haematopoietic stem cells (HSCs) are the founding cells of the adult haematopoietic system, born during ontogeny from a specialized subset of endothelium, the haemogenic endothelium (HE) via an endothelial-to-haematopoietic transition (EHT). Although recently imaged in real time, the underlying mechanism of EHT is still poorly understood. We have generated a Runx1 +23 enhancer-reporter transgenic mouse (23GFP) for the prospective isolation of HE throughout embryonic development. Here we perform functional analysis of over 1,800 and transcriptional analysis of 268 single 23GFP(+) HE cells to explore the onset of EHT at the single-cell level. We show that initiation of the haematopoietic programme occurs in cells still embedded in the endothelial layer, and is accompanied by a previously unrecognized early loss of endothelial potential before HSCs emerge. Our data therefore provide important insights on the timeline of early haematopoietic commitment.Entities:
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Year: 2013 PMID： 24326267 PMCID： PMC4362711 DOI： 10.1038/ncomms3924
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919