Mitochondrial genome of Monotrete leiurus (Osteichthyes: Tetraodontidae).

Monotrete leiurus is the only freshwater puffer founded in Nala River, which is a tributary of the Langcang-Mekong River in China. The complete mitochondrial genome sequence of the Monotrete leiurus is 16,448 bp long and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region (CR). The base composition of the genome is 29% A, 23.8% T, 31.8% C and 15.4% G and this composition shows a bias for A + T. The mitochondrial genes from Monotrete leiurus are overlapped in a total of 34 bp at seven locations and interleaved with a total of 64 bp intergenic spacers at 13 locations. The results can not only help clarify the classification problem in sibling freshwater puffers, but also provide information for their protection in the future.