Literature DB >> 2432165

Functional purification and enzymic characterization of the RNA-dependent DNA polymerase of human immunodeficiency virus.

E M Wondrak, J Löwer, R Kurth.   

Abstract

The RNA-dependent DNA polymerase (RDDP) of human immunodeficiency virus (HIV) was purified from sucrose density gradient-banded virus by four successive procedures: anion exchange chromatography, cation exchange chromatography, affinity chromatography on oligo(dT)-cellulose and adsorption chromatography on hydroxyapatite. The enzyme preparation was free of cellular DNA-dependent DNA polymerase activity. The properties of HIV RDDP were determined with a variety of template-primers. Generally, the enzyme used Mg2+ for optimal activity except with (Cm)n X (dG)12-18 as template-primer. Kinetic data (Michaelis constant, Hill coefficient) were calculated for several substrates.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 2432165     DOI： 10.1099/0022-1317-67-12-2791

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  8 in total

1.  Insertion of a peptide from MuLV RT into the connection subdomain of HIV-1 RT results in a functionally active chimeric enzyme in monomeric conformation.

Authors:  P K Pandey; N Kaushik; T T Talele; P N Yadav; V N Pandey
Journal:  Mol Cell Biochem       Date:  2001-09       Impact factor: 3.396

2.  Mutational sensitivity patterns define critical residues in the palm subdomain of the reverse transcriptase of human immunodeficiency virus type 1.

Authors:  S F Chao; V L Chan; P Juranka; A H Kaplan; R Swanstrom; C A Hutchison
Journal:  Nucleic Acids Res       Date:  1995-03-11       Impact factor: 16.971

3.  Fidelity of human immunodeficiency virus type I reverse transcriptase in copying natural DNA.

Authors:  J Weber; F Grosse
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971

4.  Isolation of human immunodeficiency virus-related simian immunodeficiency viruses from African green monkeys.

Authors:  G Kraus; A Werner; M Baier; D Binniger; F J Ferdinand; S Norley; R Kurth
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

5.  Reverse transcriptases from human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIVMAC) are susceptible to inhibition by foscarnet and 3'-azido-3'-deoxythymidine triphosphate.

Authors:  L Vrang; B Oberg; J Löwer; R Kurth
Journal:  Antimicrob Agents Chemother       Date:  1988-11       Impact factor: 5.191

6.  Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro.

Authors:  Vasudevan Achuthan; Kamlendra Singh; Jeffrey J DeStefano
Journal:  Biochemistry       Date:  2016-12-27       Impact factor: 3.162

7.  Two step binding of HIV-1 reverse transcriptase to nucleic acid substrates.

Authors:  M Kruhøffer; C Urbanke; F Grosse
Journal:  Nucleic Acids Res       Date:  1993-08-25       Impact factor: 16.971

8.  Mg2+ dependency of HIV-1 reverse transcription, inhibition by nucleoside analogues and resistance.

Authors:  Valérie Goldschmidt; Joël Didierjean; Bernard Ehresmann; Chantal Ehresmann; Catherine Isel; Roland Marquet
Journal:  Nucleic Acids Res       Date:  2006-01-03       Impact factor: 16.971
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.