Literature DB >> 2432037

Silver-enhanced colloidal gold probes as markers for scanning electron microscopy.

L Scopsi, L I Larsson, L Bastholm, M H Nielsen.   

Abstract

Silver enlargement of small colloidal gold particles has been extensively used for the light microscopical visualization of gold probes. Very recently, a few investigators have employed physical developers in electron microscopy (both pre-embedding and on-grid staining methods). We now demonstrate that physical development of small colloidal gold particles advantageously can be exploited for labelling biological surfaces in scanning electron microscopy. This novel application of silver enhancement of colloidal gold particles is characterized by a high detection efficiency. Thus, specimens are labelled with small gold probes affording high immunocytochemical efficiency but being impossible to detect with the present scanning microscopes. These particles are subsequently scanning electronmicroscopically visualized by silver enhancement.

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Year:  1986        PMID： 2432037     DOI： 10.1007/bf00492343

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  30 in total

1.  Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

Authors:  M Horisberger; J Rosset; H Bauer
Journal:  Experientia       Date:  1975-10-15

2.  Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

Authors:  C Manigley; J Roth
Journal:  J Histochem Cytochem       Date:  1985-12       Impact factor: 2.479

3.  Monoclonal antibodies to K88ab, K88ac and K88ad fimbriae from enterotoxigenic Escherichia coli.

Authors:  N T Foged; P Klemm; F Elling; S E Jorsal; J Zeuthen
Journal:  Microb Pathog       Date:  1986-02       Impact factor: 3.738

4.  Antisera to gamma-aminobutyric acid. III. Demonstration of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of cat striate cortex.

Authors:  P Somogyi; A J Hodgson
Journal:  J Histochem Cytochem       Date:  1985-03       Impact factor: 2.479

5.  Colloidal gold probes in immunocytochemistry. An optimization of their application in light microscopy by use of silver intensification procedures.

Authors:  L Scopsi; L I Larsson
Journal:  Med Biol       Date:  1986

6.  "Thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy.

Authors:  W Baschong; J M Lucocq; J Roth
Journal:  Histochemistry       Date:  1985

7.  Backscattered electron imaging for immunogold viewing by scanning electron microscope.

Authors:  M T Nava; D Soligo; E Pozzoli; G Lambertenghi-Deliliers
Journal:  J Immunol Methods       Date:  1984-05-25       Impact factor: 2.303

8.  Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence..

Authors:  L K Trejdosiewicz; M A Smolira; G M Hodges; S L Goodman; D C Livingston
Journal:  J Microsc       Date:  1981-08       Impact factor: 1.758

9.  Zonal heterogeneity of rat hepatocytes in the in vivo uptake of 17 nm colloidal gold granules.

Authors:  M J Hardonk; G Harms; J Koudstaal
Journal:  Histochemistry       Date:  1985

10.  The preparation of protein A-gold complexes with 3 nm and 15nm gold particles and their use in labelling multiple antigens on ultra-thin sections.

Authors:  J Roth
Journal:  Histochem J       Date:  1982-09
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  6 in total

1.  rRNA sequence-based scanning electron microscopic detection of bacteria.

Authors:  Takehiko Kenzaka; Ai Ishidoshiro; Nobuyasu Yamaguchi; Katsuji Tani; Masao Nasu
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

2.  Double labelling of cell surface antigens with colloidal gold markers.

Authors:  E De Harven; D Soligo; H Christensen
Journal:  Histochem J       Date:  1990-01

Review 3.  Immunogold labeling in scanning electron microscopy.

Authors:  R Hermann; P Walther; M Müller
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

4.  Effect of different fixatives on Con A surface receptors of mouse peritoneal macrophages.

Authors:  T Peschke; L Wollweber; A Gabert; K Augsten; R Stracke
Journal:  Histochemistry       Date:  1990

5.  Light- and electron-microscopical localization of calcitonin, calcitonin gene-related peptide, somatostatin and C-terminal gastrin/cholecystokinin immunoreactivities in rat thyroid.

Authors:  J Arias; L Scopsi; J A Fischer; L I Larsson
Journal:  Histochemistry       Date:  1989

6.  Schistosomiasis and in vitro transdifferentiation of murine peritoneal macrophages into fibroblastic cells.

Authors:  M Godoy; M Geuskens; E A Van Marck; R Borojevic; P Van Gansen
Journal:  Parasitol Res       Date:  1989       Impact factor: 2.289
  6 in total

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