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Literature DB >> 24318811

Two-color in situ hybridization of whole-mount mouse embryos.

Abstract

RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
RNA Probes
RNA

Year: 2014 PMID： 24318811 PMCID： PMC7724504 DOI： 10.1007/978-1-60327-292-6_2

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

14 in total

1. Cloning and expression analysis of the mouse T-box gene Tbx18.

Authors: F Kraus; B Haenig; A Kispert
Journal: Mech Dev Date: 2001-01 Impact factor: 1.882

2. Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis.

Authors: Kristin K Biris; William C Dunty; Terry P Yamaguchi
Journal: Dev Dyn Date: 2007-11 Impact factor: 3.780

3. Wnt3a links left-right determination with segmentation and anteroposterior axis elongation.

Authors: Masa-aki Nakaya; Kristin Biris; Tadasuke Tsukiyama; Shaulan Jaime; J Alan Rawls; Terry P Yamaguchi
Journal: Development Date: 2005-11-16 Impact factor: 6.868

4. Paired-related murine homeobox gene expressed in the developing sclerotome, kidney, and nervous system.

Authors: A Mansouri; Y Yokota; R Wehr; N G Copeland; N A Jenkins; P Gruss
Journal: Dev Dyn Date: 1997-09 Impact factor: 3.780

5. A mouse gene of the paired-related homeobox class expressed in the caudal somite compartment and in the developing vertebral column, kidney and nervous system.

Authors: Lorenz M Neidhardt; Andreas Kispert; B G Herrmann
Journal: Dev Genes Evol Date: 1997-11 Impact factor: 0.900

2. The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing.

Authors: Jessica Kim; Masafumi Muraoka; Hajime Okada; Atsushi Toyoda; Rieko Ajima; Yumiko Saga
Journal: PLoS Genet Date: 2022-10-05 Impact factor: 6.020

2 in total

Two-color in situ hybridization of whole-mount mouse embryos.

1. Cloning and expression analysis of the mouse T-box gene Tbx18.

2. Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis.

3. Wnt3a links left-right determination with segmentation and anteroposterior axis elongation.

4. Paired-related murine homeobox gene expressed in the developing sclerotome, kidney, and nervous system.

5. A mouse gene of the paired-related homeobox class expressed in the caudal somite compartment and in the developing vertebral column, kidney and nervous system.

6. Detection of messenger RNA by in situ hybridization to postimplantation embryo whole mounts.

7. Wnt-3a regulates somite and tailbud formation in the mouse embryo.

8. Wnt3a/beta-catenin signaling controls posterior body development by coordinating mesoderm formation and segmentation.

9. Conserved left-right asymmetry of nodal expression and alterations in murine situs inversus.

10. Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds.

1. Mesogenin 1 is a master regulator of paraxial presomitic mesoderm differentiation.

2. The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing.