Literature DB >> 24318684

Normal-phase HPLC quantitation of chlorophyll a' and phylloquinone in Photosystem I particles.

H Maeda1, T Watanabe, S Kobayashi, T Hiyama.   

Abstract

Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a' and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50-55 and contained ca. 2 molecules of Chl a' per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.

Entities:  

Year:  1993        PMID： 24318684     DOI： 10.1007/BF00014748

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  9 in total

1.  Full replacement of the function of the secondary electron acceptor phylloquinone(= vitamin K1) by non-quinone carbonyl compounds in green plant photosystem I photosynthetic reaction centers.

Authors:  S Itoh; M Iwaki
Journal:  Biochemistry       Date:  1991-06-04       Impact factor: 3.162

2.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

Review 3.  Structure, function and organization of the Photosystem I reaction center complex.

Authors:  J H Golbeck
Journal:  Biochim Biophys Acta       Date:  1987

4.  Multiple forms of P700-chlorophyll a-protein complexes from Synechococcus sp.: The iron, quinone and carotenoid contents.

Authors:  Y Takahashi; K Hirota; S Katoh
Journal:  Photosynth Res       Date:  1985-01       Impact factor: 3.573

5.  Picosecond spectroscopy of photosystem I reaction centers.

Authors:  V A Shuvalov; A V Klevanik; A V Sharkov; P G Kryukov; B Ke
Journal:  FEBS Lett       Date:  1979-11-15       Impact factor: 4.124

6.  Difference spectra and extinction coefficients of P 700 .

Authors:  T Hiyama; B Ke
Journal:  Biochim Biophys Acta       Date:  1972-04-20

7.  Electron spin resonance of chlorophyll and the origin of signal I in photosynthesis.

Authors:  J R Norris; R A Uphaus; H L Crespi; J J Katz
Journal:  Proc Natl Acad Sci U S A       Date:  1971-03       Impact factor: 11.205

8.  Characterization of a cyanobacterial photosystem I complex.

Authors:  D J Lundell; A N Glazer; A Melis; R Malkin
Journal:  J Biol Chem       Date:  1985-01-10       Impact factor: 5.157

9.  Analysis and distribution of tocopherols and quinones in the pea plant.

Authors:  J K Gaunt; B B Stowe
Journal:  Plant Physiol       Date:  1967-06       Impact factor: 8.340
  9 in total

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