Literature DB >> 24316833

Crystallization and preliminary crystallographic analysis of the chimeric protein LKB1-14-3-3ζ.

Sheng Ding1, Ruiqing Zhou, Yaqin Zhu.   

Abstract

LKB1, a tumour suppressor, plays key roles in cell polarity, cell growth and energy metabolism. 14-3-3 proteins bind to LKB1 and suppress its functions. A chimera containing 14-3-3ζ and its binding region in LKB1 was constructed and the chimeric protein LKB1-14-4-3ζ was purified and crystallized. The crystal of LKB1-14-4-3ζ diffracted to 2.9 Å resolution and belonged to space group R32, with unit-cell parameters a = b = 130.262, c = 264.960 Å. Structure determination and refinement are in progress.

Entities:  

Keywords:  14-3-3ζ; LKB1

Mesh：

Substances：

Year:  2013        PMID： 24316833      PMCID： PMC3855723          DOI： 10.1107/S1744309113029230

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  23 in total

Review 1.  14-3-3 proteins: structure, function, and regulation.

Authors:  H Fu; R R Subramanian; S C Masters
Journal:  Annu Rev Pharmacol Toxicol       Date:  2000       Impact factor: 13.820

Review 2.  Unlocking the code of 14-3-3.

Authors:  Michele K Dougherty; Deborah K Morrison
Journal:  J Cell Sci       Date:  2004-04-15       Impact factor: 5.285

3.  Growth suppression by Lkb1 is mediated by a G(1) cell cycle arrest.

Authors:  M Tiainen; A Ylikorkala; T P Mäkelä
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

4.  Growth arrest by the LKB1 tumor suppressor: induction of p21(WAF1/CIP1).

Authors:  Marianne Tiainen; Kari Vaahtomeri; Antti Ylikorkala; Tomi P Mäkelä
Journal:  Hum Mol Genet       Date:  2002-06-15       Impact factor: 6.150

5.  Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome.

Authors:  Gopal P Sapkota; Jérôme Boudeau; Maria Deak; Agnieszka Kieloch; Nick Morrice; Dario R Alessi
Journal:  Biochem J       Date:  2002-03-01       Impact factor: 3.857

6.  MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm.

Authors:  Jérôme Boudeau; Annette F Baas; Maria Deak; Nick A Morrice; Agnieszka Kieloch; Mike Schutkowski; Alan R Prescott; Hans C Clevers; Dario R Alessi
Journal:  EMBO J       Date:  2003-10-01       Impact factor: 11.598

7.  Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases.

Authors:  Seung-Pyo Hong; Fiona C Leiper; Angela Woods; David Carling; Marian Carlson
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-07       Impact factor: 11.205

8.  LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1.

Authors:  Jose M Lizcano; Olga Göransson; Rachel Toth; Maria Deak; Nick A Morrice; Jérôme Boudeau; Simon A Hawley; Lina Udd; Tomi P Mäkelä; D Grahame Hardie; Dario R Alessi
Journal:  EMBO J       Date:  2004-02-19       Impact factor: 11.598

9.  LKB1 is the upstream kinase in the AMP-activated protein kinase cascade.

Authors:  Angela Woods; Stephen R Johnstone; Kristina Dickerson; Fiona C Leiper; Lee G D Fryer; Dietbert Neumann; Uwe Schlattner; Theo Wallimann; Marian Carlson; David Carling
Journal:  Curr Biol       Date:  2003-11-11       Impact factor: 10.834

10.  The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress.

Authors:  Reuben J Shaw; Monica Kosmatka; Nabeel Bardeesy; Rebecca L Hurley; Lee A Witters; Ronald A DePinho; Lewis C Cantley
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-25       Impact factor: 11.205
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  2 in total

1.  Structure of the 14-3-3ζ-LKB1 fusion protein provides insight into a novel ligand-binding mode of 14-3-3.

Authors:  Sheng Ding; Ruiqing Zhou; Yaqin Zhu
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-08-25       Impact factor: 1.056

2.  Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.

Authors:  Nikolai N Sluchanko; Kristina V Tugaeva; Sandra J Greive; Alfred A Antson
Journal:  Sci Rep       Date:  2017-09-20       Impact factor: 4.379
  2 in total

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