| Literature DB >> 24315873 |
Jae-kyung Park1, Hoon Jang2, SeongSoo Hwang3, Eun-Jung Kim4, Dong-Ern Kim5, Keon-Bong Oh6, Dae-Jin Kwon7, Jeong-Tae Koh8, Kumi Kimura9, Hiroshi Inoue10, Won-Gu Jang11, Jeong-Woong Lee12.
Abstract
Endoplasmic reticulum (ER) stress suppresses osteoblast differentiation. Activating transcription factor (ATF) 3, a member of the ATF/cAMP response element-binding protein family of transcription factors, is induced by various stimuli including cytokines, hormones, DNA damage, and ER stress. However, the role of ATF3 in osteoblast differentiation has not been elucidated. Treatment with tunicamycin (TM), an ER stress inducer, increased ATF3 expression in the preosteoblast cell line, MC3T3-E1. Overexpression of ATF3 inhibited bone morphogenetic protein 2-stimulated expression and activation of alkaline phosphatase (ALP), an osteogenic marker. In addition, suppression of ALP expression by TM treatment was rescued by silencing of ATF3 using shRNA. Taken together, these data indicate that ATF3 is a novel negative regulator of osteoblast differentiation by specifically suppressing ALP gene expression in preosteoblasts.Entities:
Keywords: AA; AD-shATF3; ALP; ATF; Activating transcription factor 3 (ATF3); BMP2; Bone morphogenetic protein 2 (BMP2); CREB; ER; MOI; OC; Osteoblast differentiation; TM; Tunicamycin; activating transcription factor; alkaline phosphatase; anti-ATF3 shRNA; ascorbic acid 2-phosphate; bone morphogenetic protein-2; cAMP response element-binding; endoplasmic reticulum; multiplicity of infection; osteocalcin; tunicamycin; β-GP; β-glycerophosphate
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Year: 2013 PMID： 24315873 DOI： 10.1016/j.bbrc.2013.11.121
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575