OBJECTIVE: The purpose of this study was to investigate the inhibitory effect of β-catenin gene silencing on regulation of the biological behavior of neuroblastoma BE(2)C cells in vivo and in vitro. METHODS: A lentivirus, carrying β-catenin siRNA, was used to stably knockdown β-catenin expression in neuroblastoma BE(2)C cells for assessing tumor cell viability, colony formation, cell cycle distribution, apoptosis, xenograft formation, and growth in nude mice. RESULTS: Levels of β-catenin expression were markedly decreased in BE(2)C cells. Downregulation of β-catenin was concomitantly accompanied by reductions in colony formation and invasion capacity and by growth inhibition of BE(2)C cells in vitro. The mechanism appears to be a G0/G1 phase arrest and induction of apoptosis. In vivo, both tumor volume and weight of β-catenin knockdown cells were obviously reduced compared to the control and parental cells. CONCLUSION: β-Catenin knockdown could effectively control growth of neuroblastoma cells in vitro and in nude mice, suggesting that targeting β-catenin may be useful in clinical control of neuroblastoma.
OBJECTIVE: The purpose of this study was to investigate the inhibitory effect of β-catenin gene silencing on regulation of the biological behavior of neuroblastoma BE(2)C cells in vivo and in vitro. METHODS: A lentivirus, carrying β-catenin siRNA, was used to stably knockdown β-catenin expression in neuroblastoma BE(2)C cells for assessing tumor cell viability, colony formation, cell cycle distribution, apoptosis, xenograft formation, and growth in nude mice. RESULTS: Levels of β-catenin expression were markedly decreased in BE(2)C cells. Downregulation of β-catenin was concomitantly accompanied by reductions in colony formation and invasion capacity and by growth inhibition of BE(2)C cells in vitro. The mechanism appears to be a G0/G1 phase arrest and induction of apoptosis. In vivo, both tumor volume and weight of β-catenin knockdown cells were obviously reduced compared to the control and parental cells. CONCLUSION: β-Catenin knockdown could effectively control growth of neuroblastoma cells in vitro and in nude mice, suggesting that targeting β-catenin may be useful in clinical control of neuroblastoma.
Authors: Sepp R Jansen; Rian Holman; Ilja Hedemann; Ewoud Frankes; Carolina R S Elzinga; Wim Timens; Reinoud Gosens; Eveline S de Bont; Martina Schmidt Journal: J Cell Mol Med Date: 2014-09-30 Impact factor: 5.310
Authors: Anton Ogorodnikov; Michal Levin; Surendra Tattikota; Sergey Tokalov; Mainul Hoque; Denise Scherzinger; Federico Marini; Ansgar Poetsch; Harald Binder; Stephan Macher-Göppinger; Hans Christian Probst; Bin Tian; Michael Schaefer; Karl J Lackner; Frank Westermann; Sven Danckwardt Journal: Nat Commun Date: 2018-12-14 Impact factor: 14.919