PURPOSE: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. METHODS: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. RESULTS: The calibration curve was linear in the concentration range 5-60 µg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 µg/mL, respectively. CONCLUSION: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.
PURPOSE: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. METHODS: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. RESULTS: The calibration curve was linear in the concentration range 5-60 µg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 µg/mL, respectively. CONCLUSION: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.
Authors: R Nageswara Rao; K Guruprasad; Ch Gangu Naidu; B Raju; R Srinivas Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2012-02-20 Impact factor: 3.205
Authors: J C Fleishaker; K K Ryan; J M Jansat; B J Carel; D J Bell; M T Burke; N E Azie Journal: Br J Clin Pharmacol Date: 2001-05 Impact factor: 4.335
Authors: Anroop B Nair; Bandar E Al-Dhubiab; Jigar Shah; Shery Jacob; Vismay Saraiya; Mahesh Attimarad; Nagaraja SreeHarsha; Sabah H Akrawi; Tamer M Shehata Journal: Saudi Pharm J Date: 2019-12-07 Impact factor: 4.330