PURPOSE: The antimicrobial activity of doripenem in comparison of imipenem, meropenem and ertapenem among Pseudomonas aeruginosa isolated from burn and Cystic Fibrosis (CF) patients were determined. METHODS: Metallo-β-lactamase (MBL) genes in imipenem non susceptible P. aeruginosa isolates were detected using PCR method. The in vitro susceptibilities of doripenem, imipenem, meropenem and ertapenem were determined by Etests. MIC50 and MIC90 for corresponding antibiotics were determined individually in burn and CF isolates. RESULTS: Among isolates which were resistant to imipenem, 16 isolates were positive for the bla IMP gene. All isolates had no bla VIM gene. All MBL producing isolates were excluded. MIC50/MIC90 of doripenem in CF and burn isolates were 0.75/>32 and >32/>32 mg/L respectively. The corresponding values for imipenem in CF and burn isolates were 2/>32 and >32/>32 mg/L, respectively. CONCLUSION: The susceptibility rate of doripenem is higher than that of imipenem and meropenem among P.aeruginosa isolated from CF patients, whereas, there is no difference between the efficiency of doripenem and old carbapenems in non MBL producing P.aeruginosa isolates in burn patients.
PURPOSE: The antimicrobial activity of doripenem in comparison of imipenem, meropenem and ertapenem among Pseudomonas aeruginosa isolated from burn and Cystic Fibrosis (CF) patients were determined. METHODS: Metallo-β-lactamase (MBL) genes in imipenem non susceptible P. aeruginosa isolates were detected using PCR method. The in vitro susceptibilities of doripenem, imipenem, meropenem and ertapenem were determined by Etests. MIC50 and MIC90 for corresponding antibiotics were determined individually in burn and CF isolates. RESULTS: Among isolates which were resistant to imipenem, 16 isolates were positive for the bla IMP gene. All isolates had no bla VIM gene. All MBL producing isolates were excluded. MIC50/MIC90 of doripenem in CF and burn isolates were 0.75/>32 and >32/>32 mg/L respectively. The corresponding values for imipenem in CF and burn isolates were 2/>32 and >32/>32 mg/L, respectively. CONCLUSION: The susceptibility rate of doripenem is higher than that of imipenem and meropenem among P.aeruginosa isolated from CFpatients, whereas, there is no difference between the efficiency of doripenem and old carbapenems in non MBL producing P.aeruginosa isolates in burn patients.
Authors: Daniel J Wolter; Ellen Smith-Moland; Richard V Goering; Nancy D Hanson; Philip D Lister Journal: Diagn Microbiol Infect Dis Date: 2004-09 Impact factor: 2.803
Authors: M G Vossen; J M Wenisch; A Maier-Salamon; A Fritsch; K Saria; C Zuba; S Jilch; R Lemmerer; M Unger; U Jaehde; W Jäger; F Thalhammer Journal: Antimicrob Agents Chemother Date: 2015-12-28 Impact factor: 5.191
Authors: Z Hojabri; M Mirmohammadkhani; F Kamali; K Ghassemi; S Taghavipour; O Pajand Journal: Eur J Clin Microbiol Infect Dis Date: 2017-05-27 Impact factor: 3.267