Far-red mediated polyribosome formation in radish cotyledons : Effect of endogenous ribonucleases on polyribosome recovery.

The total ribosome content of radish cotyledons increases during the first 2-3 days of germination both in darkness and under far-red light irradiation; ribonuclease activity is not under phytochrome control during this period. Changes in ribonuclease activity interfere with the analysis of the polyribosomal population. A maximal ratio of polysomes to monosomes is observed 12 h after the onset of far-red light and then it decreases. A 12 h far-red irradiation stimulates the in vivo incorporation of amino acids into proteins. This stimulation persists when seedlings are transferred for 4 h to the dark.