Literature DB >> 24310042

Gel isoelectric focusing of wheat alcohol dehydrogenase.

O Fejér1, K Orosz-Fejér, A Belea.   

Abstract

Alcohol dehydrogenase (ADH) of different hexaploid wheat subspecies and varieties was investigated by isoelectric focusing in polyacrylamide gels. With this technique six ADH isoenzymes can be separated, while by the standard electrophoretic technique only three are visible. The ADH pattern revealed by isoelectric focusing is in full accordance with the hypothesis that the active ADH isozymes in hexaploid wheat are dimers composed of six possible combinations of subunits coded by triplicate structural genes.

Entities:  

Year:  1979        PMID: 24310042     DOI: 10.1007/BF00265706

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN: 0040-5752            Impact factor:   5.699


  9 in total

1.  Isoelectric fractionation of proteins on polyacrylamide gels.

Authors:  J S. Fawcett
Journal:  FEBS Lett       Date:  1968-07       Impact factor: 4.124

2.  Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat.

Authors:  G E Hart
Journal:  Proc Natl Acad Sci U S A       Date:  1970-08       Impact factor: 11.205

Review 3.  Isoelectric points of proteins: a table.

Authors:  D Malamud; J W Drysdale
Journal:  Anal Biochem       Date:  1978-06-01       Impact factor: 3.365

4.  Activity tests of alcohol dehydrogenases in wheat, rye and triticale.

Authors:  F Leibenguth
Journal:  Experientia       Date:  1977-11-15

5.  Isoelectric focusing in polyacrylamide gels.

Authors:  G Dale; A L Latner
Journal:  Lancet       Date:  1968-04-20       Impact factor: 79.321

6.  Isoelectric focusing of insulins in polyacrylamide gel.

Authors:  K D Kohnert; E Schmid; H Zühlke; H Fiedler
Journal:  J Chromatogr       Date:  1973-02-07

7.  Genetic control of alcohol dehydrogenase isozymes in Triticum dicoccum.

Authors:  G E Hart
Journal:  Biochem Genet       Date:  1969-12       Impact factor: 1.890

8.  Heterogeneity of horse liver alcohol dehydrogenase. Purification and characterization of multiple molecular forms.

Authors:  U M Lutstorf; P M Schürch; J P von Wartburg
Journal:  Eur J Biochem       Date:  1970-12

9.  Gel isoelectric focusing of mouse lactate dehydrogenase: heterogeneity of the isoenzymes A4 and X4.

Authors:  J Klose; H Spielmann
Journal:  Biochem Genet       Date:  1975-10       Impact factor: 1.890

  9 in total
  1 in total

1.  Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 2. Genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs.

Authors:  M Zivy; H Thiellement; D de Vienne; J P Hofmann
Journal:  Theor Appl Genet       Date:  1984-07       Impact factor: 5.699

  1 in total

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