OBJECTIVE: We assessed the metabolic characteristics of high-fat-diet-resistant (DR) rats. METHODS: Body weight, energy intake, locomotor activity, oxygen consumption, plasma leptin and lipid levels, size of visceral-fat adipocytes, and mRNA levels of genes related to lipid metabolism were measured in control rats fed standard chow and in obesity-prone (high-fat-diet-induced obesity, DIO) and DR rats fed a high-fat diet. Glucose tolerance and insulin tolerance tests were also performed. RESULTS: DIO rats gained weight more rapidly than did DR and control rats; DR rats gained less weight than did DIO rats despite similar energy intake. Energy expenditure did not differ among the three groups. The diameter of visceral-fat adipocytes was similar in DR and control rats. mRNA levels of genes involved in lipogenesis, such as fatty acid synthase and acetyl-CoA carboxylase, tended to be lower in DR than in control and DIO rats, whereas those of carnitine palmitoyltransferase 1a, which is involved in fatty acid β-oxidation, were greater in DR rats than in the other groups. DIO rats showed hyperinsulinemia and glucose intolerance, whereas DR rats had high sensitivity to insulin. CONCLUSION: DR rats show suppression of lipogenesis and acceleration of fatty acid β-oxidation in the visceral fat. These characteristics likely contribute to the anti-obesity phenotype of DR rats.
OBJECTIVE: We assessed the metabolic characteristics of high-fat-diet-resistant (DR) rats. METHODS: Body weight, energy intake, locomotor activity, oxygen consumption, plasma leptin and lipid levels, size of visceral-fat adipocytes, and mRNA levels of genes related to lipid metabolism were measured in control rats fed standard chow and in obesity-prone (high-fat-diet-induced obesity, DIO) and DR rats fed a high-fat diet. Glucose tolerance and insulin tolerance tests were also performed. RESULTS: DIO rats gained weight more rapidly than did DR and control rats; DR rats gained less weight than did DIO rats despite similar energy intake. Energy expenditure did not differ among the three groups. The diameter of visceral-fat adipocytes was similar in DR and control rats. mRNA levels of genes involved in lipogenesis, such as fatty acid synthase and acetyl-CoA carboxylase, tended to be lower in DR than in control and DIO rats, whereas those of carnitine palmitoyltransferase 1a, which is involved in fatty acid β-oxidation, were greater in DR rats than in the other groups. DIO rats showed hyperinsulinemia and glucose intolerance, whereas DR rats had high sensitivity to insulin. CONCLUSION: DR rats show suppression of lipogenesis and acceleration of fatty acid β-oxidation in the visceral fat. These characteristics likely contribute to the anti-obesity phenotype of DR rats.
Authors: Rosângela Maria Lopes Sousa; Nathalee Liberal Xavier Ribeiro; Bruno Araújo Serra Pinto; Jonas Rodrigues Sanches; Mariana Uchôa da Silva; Caio Fernando Ferreira Coêlho; Lucas Martins França; José Albuquerque de Figueiredo Neto; Antonio Marcus de Andrade Paes Journal: Nutr Metab (Lond) Date: 2018-07-24 Impact factor: 4.169
Authors: Felipe Gonçalves Dos Santos de Sá; Ana Paula Lima-Leopoldo; Bruno Barcellos Jacobsen; Artur Junio Togneri Ferron; Wagner Muller Estevam; Dijon Henrique Salomé Campos; Edson Castardeli; Márcia Regina Holanda da Cunha; Antonio Carlos Cicogna; André Soares Leopoldo Journal: Arq Bras Cardiol Date: 2015-10-27 Impact factor: 2.000