| Literature DB >> 2430580 |
E V Grishin, T M Volkova, A S Arsen'ev, O S Reshetova, V V Onoprienko.
Abstract
Argiopine, a compound capable of blocking the glutamate-activated ion channels in experiments with glutamatergic synapses of blowfly larvae, was isolated from the venom of spider Argiope lobata. Argiopine-receptor complex has KD 6,7 X 10(-7) M. The venom solution was treated with 60% ethanol, centrifuged and supernatant was subjected to reversed-phase HPLC in the acetonitrile concentration gradient. Argiopine structure was established using 1H- and 13C-NMR spectroscopy, mass spectrometry, elemental and amino acid analyses. Argiopine (molecular mass 636) consists of arginine (free alpha-NH2) linke with polyamine--NH (CH2)3NH(CH2)3NH(CH2)5NH--through a peptide bond. The polyamine is connected to the alpha-carboxyl group of asparagine whose alpha-amino group is linked to 2,4-dihydroxyphenylacetic acid.Entities:
Mesh：
Substances：
Year: 1986 PMID： 2430580
Source DB: PubMed Journal: Bioorg Khim ISSN： 0132-3423