Literature DB >> 2430303

Monoclonal antibodies to glutamic acid decarboxylase.

D I Gottlieb, Y C Chang, J E Schwob.   

Abstract

Five monoclonal antibodies that recognize chicken brain glutamic acid decarboxylase (GAD) have been selected and designated GAD-1 to -5. GAD-1 to -5 were selected on the basis of their ability to immunoprecipitate active GAD from crude brain extracts. GAD-1 recognizes an epitope that is conserved in many vertebrates; the epitope recognized by GAD-5 is restricted to the chicken. Radioimmunoassays with GAD-1 indicate that GAD is highly enriched in brain relative to other tissues. GAD was localized immunocytochemically with GAD-1 and GAD-2 in rat cerebellum, spinal cord, and retina. The staining pattern is in agreement with that obtained previously with polyclonal antisera to GAD. GAD from the chicken brain was purified by chromatography on an immunoaffinity column made of GAD-1. NaDodSO4/PAGE analysis of the immunoaffinity-purified GAD fractions shows a major band of 59 kDa and minor bands at 63 and 54 kDa.

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Year:  1986        PMID： 2430303      PMCID： PMC387021          DOI： 10.1073/pnas.83.22.8808

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

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Authors:  J C Jensenius; A F Williams
Journal:  Eur J Immunol       Date:  1974-02       Impact factor: 5.532

4.  Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy.

Authors:  I W McLean; P K Nakane
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

5.  Purification and characterization of glutamate decarboxylase from mouse brain.

Authors:  J Y Wu; T Matsuda; E Roberts
Journal:  J Biol Chem       Date:  1973-05-10       Impact factor: 5.157

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Journal:  Eur J Biochem       Date:  1978-05

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Authors:  K Saito; R Barber; J Wu; T Matsuda; E Roberts; J E Vaughn
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205

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Authors:  P B Molinoff; E A Kravitz
Journal:  J Neurochem       Date:  1968-05       Impact factor: 5.372
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  30 in total

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Journal:  Pharm Res       Date:  1999-07       Impact factor: 4.200

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Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

6.  Serotonin-driven long-range inhibitory connections in the cerebellar cortex.

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8.  Synaptic and extrasynaptic localization of brain-derived neurotrophic factor and the tyrosine kinase B receptor in cultured hippocampal neurons.

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9.  Localization of GAD-like immunoreactivity in the pancreas and stomach of the rat and mouse.

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