Y-H Yan1, P Yi, Y-R Zheng, L-L Yu, J Han, X-M Han, L Li. 1. Department of Obstetrics and Gynecology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China. 172535131@qq.com.
Abstract
OBJECTIVES: Preeclampsia is a complication of pregnancy that severely threatens the health of the mother and infant, yet the mechanism of pathogenesis remains unclear. In this article, gene array technology was applied to identify the genes related to the pathogenesis of preeclampsia, and to explore the regulatory effect of epigenetic modification by on these genes. PATIENTS AND METHODS: Placental tissue of preeclampsia patients was collected, and DNA methylation arrays and gene expression microarrays were used to identify the genes. The effect of methylation on the regulation of genes related to the pathogenesis of preeclampsia was also investigated. RESULTS: The expression levels of more than ten genes were found to be significantly altered in the placental tissue of patients with preeclampsia as measured by gene expression microarray. This study also identified more than ten genes with notable changes in expression level as well as methylation level. The gene expression of CUEDC1 and DHX34 were verified in this study and the findings were consistent with previous reports. CONCLUSIONS: Our research indicates that the occurrence of preeclampsia is correlates closely with differences in the expression of specific genes, which may be regulated through methylation.
OBJECTIVES: Preeclampsia is a complication of pregnancy that severely threatens the health of the mother and infant, yet the mechanism of pathogenesis remains unclear. In this article, gene array technology was applied to identify the genes related to the pathogenesis of preeclampsia, and to explore the regulatory effect of epigenetic modification by on these genes. PATIENTS AND METHODS: Placental tissue of preeclampsia patients was collected, and DNA methylation arrays and gene expression microarrays were used to identify the genes. The effect of methylation on the regulation of genes related to the pathogenesis of preeclampsia was also investigated. RESULTS: The expression levels of more than ten genes were found to be significantly altered in the placental tissue of patients with preeclampsia as measured by gene expression microarray. This study also identified more than ten genes with notable changes in expression level as well as methylation level. The gene expression of CUEDC1 and DHX34 were verified in this study and the findings were consistent with previous reports. CONCLUSIONS: Our research indicates that the occurrence of preeclampsia is correlates closely with differences in the expression of specific genes, which may be regulated through methylation.
Authors: Haley R Eidem; William E Ackerman; Kriston L McGary; Patrick Abbot; Antonis Rokas Journal: BMC Med Genomics Date: 2015-06-05 Impact factor: 3.063
Authors: Sandra G Heil; Emilie M Herzog; Pieter H Griffioen; Bertrand van Zelst; Sten P Willemsen; Yolanda B de Rijke; Regine P M Steegers-Theunissen; Eric A P Steegers Journal: PLoS One Date: 2019-12-30 Impact factor: 3.240