Literature DB >> 24301997

Membrane vesicles fromSynechocystis 6803 showing proton and electron transport and high ATP synthase activities.

M J Scholts1, P Aardewijn, H S Van Walraven.   

Abstract

A simple procedure for the preparation of well-coupled and stable membrane vesicles from the transformable cyanobacteriumSynechocystis 6803 is described with the primary aim of producing vesicles suitable for the study of photosynthetic electron transport and phosphorylation. Spheroplasts were obtained from the cyanobacterium by lysozyme treatment and stored untill prior to measurement, thylakoid vesicles were obtained by osmotic shock. These vesicles showed very high and stable ATP synthesis rates either driven by light or by acid-base transition, and also performed light-induced ATP hydrolysis and linear electron transport. Formation of a proton gradient is studied by aminoacridines.

Entities:  

Year:  1996        PMID： 24301997     DOI： 10.1007/BF02184291

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  10 in total

1.  ATP synthase from a cyanobacterial Synechocystis 6803 mutant containing the regulatory segment of the chloroplast gamma subunit shows thiol modulation.

Authors:  B E Krenn; P Aardewijn; H S Van Walraven; S Werner-Grüne; H Strotmann; R Kraayenhof
Journal:  Biochem Soc Trans       Date:  1995-11       Impact factor: 5.407

2.  DNA transformation.

Authors:  R D Porter
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

3.  Mutagenesis of the b'-subunit of Synechocystis sp. PCC 6803 ATP-synthase.

Authors:  H Lill; D Steinemann; N Nelson
Journal:  Biochim Biophys Acta       Date:  1994-03-08

4.  Insertion of a "chloroplast-like" regulatory segment responsible for thiol modulation into gamma-subunit of F0F1-ATPase of the cyanobacterium Synechocystis 6803 by mutagenesis of atpC.

Authors:  S Werner-Grüne; D Gunkel; J Schumann; H Strotmann
Journal:  Mol Gen Genet       Date:  1994-07-25

5.  Photochemical activity and components of membrane preparations from blue-green algae. I. Coexistence of two photosystems in relation to chlorophyll a and removal of phycocyanin.

Authors:  D I Arnon; B D McSwain; H Y Tsujimoto; K Wada
Journal:  Biochim Biophys Acta       Date:  1974-08-23

6.  Organization and sequences of genes for the subunits of ATP synthase in the thermophilic cyanobacterium Synechococcus 6716.

Authors:  H S Van Walraven; R Lutter; J E Walker
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

7.  Glycinebetaine enhances and stabilizes the evolution of oxygen and the synthesis of ATP by cyanobacterial thylakoid membranes.

Authors:  M D Mamedov; H Hayashi; H Wada; P S Mohanty; G C Papageorgiou; N Murata
Journal:  FEBS Lett       Date:  1991-12-09       Impact factor: 4.124

8.  Studies on the structure and conformation of yeast mitochondrial ATPase using aurovertin and methanol as probes.

Authors:  E Stutterheim; M A Henneke; J A Berden
Journal:  Biochim Biophys Acta       Date:  1980-10-03

9.  Modulation of the proton-translocation stoichiometry of H(+)-ATP synthases in two phototrophic prokaryotes by external pH.

Authors:  B E Krenn; H S Van Walraven; M J Scholts; R Kraayenhof
Journal:  Biochem J       Date:  1993-09-15       Impact factor: 3.857

10.  On the activation mechanism of the H(+)-ATP synthase and unusual thermodynamic properties in the alkalophilic cyanobacterium Spirulina platensis.

Authors:  R H Bakels; H S van Walraven; K Krab; M J Scholts; R Kraayenhof
Journal:  Eur J Biochem       Date:  1993-05-01
  10 in total
  3 in total

1.  Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973.

Authors:  Justin Ungerer; Kristen E Wendt; John I Hendry; Costas D Maranas; Himadri B Pakrasi
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-08       Impact factor: 11.205

2.  pH-dependent Ca2+ binding to the F0 c-subunit affects proton translocation of the ATP synthase from Synechocystis 6803.

Authors:  Hendrika S Van Walraven; Marijke J C Scholts; Stanislav D Zakharov; Ruud Kraayenhof; Richard A Dilley
Journal:  J Bioenerg Biomembr       Date:  2002-12       Impact factor: 2.945

3.  Bioenergetic responses of Synechocystis 6803 fatty acid desaturase mutants at low temperatures.

Authors:  R A Dilley; Y Nishiyama; Z Gombos; N Murata
Journal:  J Bioenerg Biomembr       Date:  2001-04       Impact factor: 2.945
  3 in total

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