Literature DB >> 24300106

Calcium phosphate transfection of primary hippocampal neurons.

Miao Sun1, Laura P Bernard, Victoria L Dibona, Qian Wu, Huaye Zhang.   

Abstract

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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Year:  2013        PMID： 24300106      PMCID： PMC3989506          DOI： 10.3791/50808

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  10 in total

1.  Fast, convenient, and effective method to transiently transfect primary hippocampal neurons.

Authors:  M Köhrmann; W Haubensak; I Hemraj; C Kaether; V J Lessmann; M A Kiebler
Journal:  J Neurosci Res       Date:  1999-12-15       Impact factor: 4.164

2.  Chemically controlled formation of a DNA/calcium phosphate coprecipitate: application for transfection of mature hippocampal neurons.

Authors:  Bernhard Goetze; Barbara Grunewald; Simona Baldassa; Michael Kiebler
Journal:  J Neurobiol       Date:  2004-09-15

Review 3.  Transfection techniques for neuronal cells.

Authors:  Daniela Karra; Ralf Dahm
Journal:  J Neurosci       Date:  2010-05-05       Impact factor: 6.167

4.  High Ca2+-phosphate transfection efficiency in low-density neuronal cultures.

Authors:  Min Jiang; Gong Chen
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5.  High-efficiency transfection of mammalian neurons via nucleofection.

Authors:  Manuel Zeitelhofer; John P Vessey; Yunli Xie; Fabian Tübing; Sabine Thomas; Michael Kiebler; Ralf Dahm
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

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Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Calcium phosphate transfection of DNA into neurons in primary culture.

Authors:  H Dudek; A Ghosh; M E Greenberg
Journal:  Curr Protoc Neurosci       Date:  2001-05

8.  Lentiviral delivery of RNAi in hippocampal neurons.

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Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

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Authors:  Kambiz N Alavian; Hongmei Li; Leon Collis; Laura Bonanni; Lu Zeng; Silvio Sacchetti; Emma Lazrove; Panah Nabili; Benjamin Flaherty; Morven Graham; Yingbei Chen; Shanta M Messerli; Maria A Mariggio; Christoph Rahner; Ewan McNay; Gordon C Shore; Peter J S Smith; J Marie Hardwick; Elizabeth A Jonas
Journal:  Nat Cell Biol       Date:  2011-09-18       Impact factor: 28.824

10.  Modulation of synaptic function by VAC14, a protein that regulates the phosphoinositides PI(3,5)P₂ and PI(5)P.

Authors:  Yanling Zhang; Amber J McCartney; Sergey N Zolov; Cole J Ferguson; Miriam H Meisler; Michael A Sutton; Lois S Weisman
Journal:  EMBO J       Date:  2012-07-27       Impact factor: 11.598
  10 in total
  14 in total

1.  Organic Anion Transporter 1 Deficiency Accelerates Learning and Memory Impairment in tg2576 Mice by Damaging Dendritic Spine Morphology and Activity.

Authors:  Xinlin Wu; Jianqing Zhang; Heng Liu; Yansheng Mian; Birong Liang; Hongbo Xie; Shijun Zhang; Baoguo Sun; Houming Zhou
Journal:  J Mol Neurosci       Date:  2015-03-01       Impact factor: 3.444

2.  Par3 and aPKC regulate BACE1 endosome-to-TGN trafficking through PACS1.

Authors:  Miao Sun; Huaye Zhang
Journal:  Neurobiol Aging       Date:  2017-09-21       Impact factor: 4.673

3.  Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

Authors:  Qian Wu; Miao Sun; Laura P Bernard; Huaye Zhang
Journal:  J Biol Chem       Date:  2017-08-08       Impact factor: 5.157

4.  Par3 regulates polarized convergence between APP and BACE1 in hippocampal neurons.

Authors:  Miao Sun; Chengyu Huang; Hui Wang; Huaye Zhang
Journal:  Neurobiol Aging       Date:  2019-01-30       Impact factor: 4.673

5.  Gene delivery using calcium phosphate nanoparticles: Optimization of the transfection process and the effects of citrate and poly(l-lysine) as additives.

Authors:  Mohammed A Khan; Victoria M Wu; Shreya Ghosh; Vuk Uskoković
Journal:  J Colloid Interface Sci       Date:  2016-03-05       Impact factor: 8.128

6.  Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters.

Authors:  C G Piett; T J Pecen; D J Laverty; Z D Nagel
Journal:  Nat Protoc       Date:  2021-08-06       Impact factor: 17.021

7.  MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

Authors:  Laura P Bernard; Huaye Zhang
Journal:  PLoS One       Date:  2015-05-01       Impact factor: 3.240

8.  Optimizing conditions for calcium phosphate mediated transient transfection.

Authors:  Ling Guo; Liyang Wang; Ronghua Yang; Rui Feng; Zhongguang Li; Xin Zhou; Zhilong Dong; George Ghartey-Kwansah; MengMeng Xu; Miyuki Nishi; Qi Zhang; Williams Isaacs; Jianjie Ma; Xuehong Xu
Journal:  Saudi J Biol Sci       Date:  2017-02-11       Impact factor: 4.219

9.  A Simple and Efficient In Vivo Non-viral RNA Transfection Method for Labeling the Whole Axonal Tree of Individual Adult Long-Range Projection Neurons.

Authors:  César Porrero; Javier Rodríguez-Moreno; José I Quetglas; Cristian Smerdou; Takahiro Furuta; Francisco Clascá
Journal:  Front Neuroanat       Date:  2016-03-18       Impact factor: 3.856

10.  Depression is Associated with CRP SNPs in Patients with Family History.

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Journal:  Transl Neurosci       Date:  2017-12-29       Impact factor: 1.757
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