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Literature DB >> 2429580

Antibody probing of western blots which have been stained with india ink.

Abstract

India ink can be used to stain proteins bound to nitrocellulose. Subsequently, specific proteins can be identified with antibodies and 125I-protein A. In most cases, india ink did not significantly inhibit detection with antibody, nor was the ink washed off during the antibody incubation steps. Using this method, a direct comparison of antibody-reactive protein and total protein can be made with the same replica.

Entities: Chemical

Mesh：

Substances：

Year: 1986 PMID： 2429580 DOI： 10.1016/0003-2697(86)90259-9

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

22 in total

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2. p180, a novel recycling transmembrane glycoprotein with restricted cell type expression.

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3. Subunit structure, expression, and function of NAD(H)-specific isocitrate dehydrogenase in Saccharomyces cerevisiae.

Authors: D A Keys; L McAlister-Henn
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4. Purification and characterization of heparin-binding growth factors from porcine uterus.

Authors: D R Brigstock; R B Heap; P J Barker; K D Brown
Journal: Biochem J Date: 1990-02-15 Impact factor: 3.857

5. The antioxidant vitamin E modulates amyloid beta-peptide-induced creatine kinase activity inhibition and increased protein oxidation: implications for the free radical hypothesis of Alzheimer's disease.

Authors: S M Yatin; M Aksenov; D A Butterfield
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6. Temporal relations among amyloid beta-peptide-induced free-radical oxidative stress, neuronal toxicity, and neuronal defensive responses.

Authors: S M Yatin; M Aksenova; M Aksenov; W R Markesbery; T Aulick; D A Butterfield
Journal: J Mol Neurosci Date: 1998-12 Impact factor: 3.444

Antibody probing of western blots which have been stained with india ink.

Review 1. Protein (western) blotting.

2. p180, a novel recycling transmembrane glycoprotein with restricted cell type expression.

3. Subunit structure, expression, and function of NAD(H)-specific isocitrate dehydrogenase in Saccharomyces cerevisiae.

4. Purification and characterization of heparin-binding growth factors from porcine uterus.

5. The antioxidant vitamin E modulates amyloid beta-peptide-induced creatine kinase activity inhibition and increased protein oxidation: implications for the free radical hypothesis of Alzheimer's disease.

6. Temporal relations among amyloid beta-peptide-induced free-radical oxidative stress, neuronal toxicity, and neuronal defensive responses.

7. Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas.

8. Detection of fos protein during osteogenesis by monoclonal antibodies.

9. An alternative approach to somatic cell gene therapy.

10. Differences in phorbol-ester-induced down-regulation of protein kinase C between cell lines.