BACKGROUND: The high-throughput analysis of strontium and calcium in plasma, tissue and bone (femur) using inductively coupled plasma-MS is described. Method validation, the results and experience obtained during sample analysis are highlighted. RESULTS: The different matrices were destructed with concentrated nitric acid by heating at 60°C overnight. Using this approach it was possible to analyze large numbers of samples in parallel. CONCLUSION: Inductively coupled plasma-MS proved to be a highly sensitive, robust and efficient technique for the analysis of several different biological samples. A total of 6767 samples were analyzed, and the performance of the method was illustrated by the fact that only 0.2% of the samples had to be reanalyzed due to anomalous results and ISR for all matrices fulfilled the acceptance criteria.
BACKGROUND: The high-throughput analysis of strontium and calcium in plasma, tissue and bone (femur) using inductively coupled plasma-MS is described. Method validation, the results and experience obtained during sample analysis are highlighted. RESULTS: The different matrices were destructed with concentrated nitric acid by heating at 60°C overnight. Using this approach it was possible to analyze large numbers of samples in parallel. CONCLUSION: Inductively coupled plasma-MS proved to be a highly sensitive, robust and efficient technique for the analysis of several different biological samples. A total of 6767 samples were analyzed, and the performance of the method was illustrated by the fact that only 0.2% of the samples had to be reanalyzed due to anomalous results and ISR for all matrices fulfilled the acceptance criteria.
Authors: Ju Lan Chun; Han Tae Bang; Sang Yun Ji; Jin Young Jeong; Minji Kim; Byeonghyeon Kim; Sung Dae Lee; Yoo Kyung Lee; Kondreddy Eswar Reddy; Ki Hyun Kim Journal: J Anim Sci Technol Date: 2020-01-31