Literature DB >> 24293828

Production and Application of Polyclonal Antibodies Against Recombinant Capsid Protein of Extra Small Virus of Macrobrachium rosenbergii.

V Neethi1, N Sivakumar, Kundan Kumar, K V Rajendran, M Makesh.   

Abstract

Macrobrachium rosenbergii nodavirus along with a satellite virus, extra small virus (XSV) causes white tail disease (WTD) in the giant freshwater prawn M. rosenbergii. Infected M. rosenbergii postlarvae were collected from a hatchery in Kakinada, Andhra Pradesh. The gene coding the capsid protein of XSV was cloned in a bacterial expression vector pRSET A and the recombinant protein was expressed in Escherichia coli BL21(DE3)pLysS cells. The recombinant protein was purified by Nickel affinity chromatography. Polyclonal antibodies were produced in mice against the recombinant protein and the antibodies reacted specifically with the recombinant protein and XSV in WTD-infected tissues. This is the first report of detection of XSV using antibodies against recombinant capsid protein.

Entities:  

Keywords:  Capsid protein; Macrobrachium rosenbergii; Polyclonal antibodies; White tail disease; XSV

Year:  2012        PMID： 24293828      PMCID： PMC3550785          DOI： 10.1007/s13337-012-0090-3

Source DB:  PubMed          Journal:  Indian J Virol        ISSN： 0970-2822


  12 in total

1.  White tail disease of the giant freshwater prawn Macrobrachium rosenbergii in Thailand.

Authors:  Kalidoss Yoganandhan; Manee Leartvibhas; Supamas Sriwongpuk; Chalor Limsuwan
Journal:  Dis Aquat Organ       Date:  2006-04-06       Impact factor: 1.802

2.  White tail disease of the giant freshwater prawn, Macrobrachium rosenbergii: separation of the associated virions and characterization of MrNV as a new type of nodavirus.

Authors:  J-R Bonami; Z Shi; D Qian; J Sri Widada
Journal:  J Fish Dis       Date:  2005-01       Impact factor: 2.767

3.  Quantitative relationship of two viruses (MrNV and XSV) in white-tail disease of Macrobrachium rosenbergii.

Authors:  Huajun Zhang; Jianmin Wang; Junfa Yuan; Lijuan Li; Jianhong Zhang; Jean-Robert Bonami; Zhengli Shi
Journal:  Dis Aquat Organ       Date:  2006-07-11       Impact factor: 1.802

4.  RT-PCR amplification and sequence analysis of extra small virus associated with white tail disease of Macrobrachium rosenbergii (de Man) cultured in Taiwan.

Authors:  C S Wang; J S Chang; H H Shih; S N Chen
Journal:  J Fish Dis       Date:  2007-03       Impact factor: 2.767

5.  Rapid detection of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), the pathogenic agents of white tail disease of Macrobrachium rosenbergii (De Man), by loop-mediated isothermal amplification.

Authors:  D Pillai; J-R Bonami; J Sri Widada
Journal:  J Fish Dis       Date:  2006-05       Impact factor: 2.767

6.  Simultaneous detection of Macrobrachium rosenbergii nodavirus and extra small virus by a single tube, one-step multiplex RT-PCR assay.

Authors:  K Yoganandhan; J Sri Widada; J R Bonami; A S Sahul Hameed
Journal:  J Fish Dis       Date:  2005-02       Impact factor: 2.767

7.  Genome-based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, Macrobrachium rosenbergii dot-blot, in situ hybridization and RT-PCR.

Authors:  J Sri Widada; S Durand; I Cambournac; D Qian; Z Shi; E Dejonghe; V Richard; J R Bonami
Journal:  J Fish Dis       Date:  2003-10       Impact factor: 2.767

8.  Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii.

Authors:  D Qian; Z Shi; S Zhang; Z Cao; W Liu; L Li; Y Xie; I Cambournac; J-R Bonami
Journal:  J Fish Dis       Date:  2003-09       Impact factor: 2.767

9.  A sandwich enzyme linked immunosorbent assay (S-ELISA) for detection of MrNV in the giant freshwater prawn, Macrobrachium rosenbergii (de Man).

Authors:  B Romestand; J R Bonami
Journal:  J Fish Dis       Date:  2003-02       Impact factor: 2.767

10.  Macrobrachium rosenbergii nodavirus disease (white tail disease) in Australia.

Authors:  Leigh Owens; Kathy La Fauce; Karen Juntunen; Orachun Hayakijkosol; Chaoshu Zeng
Journal:  Dis Aquat Organ       Date:  2009-07-23       Impact factor: 1.802
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